BLOOD CULTURE (088-0431)
Synonyms: Culture, bacterial, blood
CPT 4 Code: 87040
Test Order Mnemonic: C BLDN
Test Includes: Aerobic and anaerobic bacteria and yeast; antimicrobial testing when appropriate
Request Form: Microbiology
Patient Preparation: Cleanse skin with Medi-Flex kit provided with the blood culture bottles.
Collection: Do not touch phlebotomy site after cleansing to prevent false positive culture growth. Use a syringe or butterfly to collect 13-17 ml of blood. Pediatric patients, collect 1-3mL of blood. Inject blood into the alcohol-cleaned aerobic and anaerobic blood culture vials. Inject 8 ml into the aerobic bottle; inject 5 ml into the anaerobic bottle. If a pediatric bottle is used, inject it with 1-3 ml of blood (min 0.5ml).
Storage Instructions: Store at room temperature or incubate at 35░C until transport.
Causes for Rejection: Refrigerated vials, submission of vials other than Bactec vials
Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough.
Turnaround Time: Preliminary reports after 24 and 48 hours, final report after 7 days
Special Instructions: Collect specimens using aseptic technique since contamination may lead to false positive results. Collect two sets of cultures per febrile episode. Positive culture results are considered a critical value and notification will be performed upon recognition of the positive result. For pediatric patients, there is a Pediatric Bactec bottle available. Inoculate this bottle with 1-3 ml of blood. DO NOT COVER THE BAR CODE LABEL.
Specimen: Blood, venous or line (indicate line site)
Volume: Aerobic bottle 8 ml, Anaerobic bottle 5 ml, Pediatric 1-3ml
Minimum Volume: Pediatric, 0.5 ml
Container: Bactec Bottles: Aerobic, blue; Anaerobic: yellow; Pediatric: pink
Critical Values: Positive
Test Method: Bactec 9240
Revised 6/22/2011 by T. Friloux