BRUCELLA CULTURE (088-0431)
Synonyms: Blood Culture for Brucella
CPT 4 Code: 87040
Test Order Mnemonic: C BLDN, Brucella blood culture
Request Form: Microbiology
Collection: Collect the specimen in the same manner as for a routine blood culture but inoculate only the aerobic vial. Follow instructions in the Medi-flex kit. Withdraw 8-10 mL of blood with a syringe and inject into an alcohol-cleaned Bactec aerobic vial.
Storage Instructions: Room temperature. Do not refrigerate
Causes for Rejection: Improper collection
Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough.
Turnaround Time: Preliminary report after 1 day, final report after 21 days
Special Instructions: Indicate on the requisition that Brucella is suspected so the laboratory will incubate this culture for 21 days. When submitting samples other than blood in a blood culture bottle, indicate so on the requisition slip.
Specimen: Blood (other acceptable samples are bone marrow or tissues)
Volume: 10 ml
Minimum Volume: 8 ml
Container: Bactec blood vial, available through Materials Management
Critical Values: Culture positive
Test Method: Bactec 9240
Revised/reviewed 6/22/2011 by T. Friloux