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BRUCELLA CULTURE (088-0431) Synonyms: Blood Culture for Brucella CPT 4 Code: 87040 Test Order Mnemonic: C BLDN Lab: Bacteriology Request Form: Microbiology Collection: Collect the specimen in the same manner as for a routine blood culture using only the aerobic vial. Follow instructions in the Medi-flex kit. Withdraw 8-10 mL of blood with a syringe and inject into an alcohol-cleaned Bactec aerobic vial. Storage Instructions: Room temperature. Do not refrigerate Causes for Rejection: Improper collection Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough. Turnaround Time: Preliminary report after 1 day, final report after 21 days Special Instructions: Indicate on the requisition that Brucella is suspected so the laboratory will incubate this culture for 21 days. When submitting samples other than blood in a blood culture bottle, indicate so on the requisition slip. Specimen: Blood (other acceptable samples are bone marrow or tissues) Volume: 10 ml Minimum Volume: 8 ml Container: Bactec blood vial, available through Materials Management Critical Values: Culture positive Test Method: Bactec 9240 Revised/reviewed 07/25/2008 by T. Friloux |
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