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Chlamydia Trachomatis (ct) amplified assay: (088-0093)

Clinical Indication:

 Detection of Chlamydia trachomatis in urine, endocervical, urethral, vaginal, oral and rectal specimens.

Test Mnemonic:

 V NCAA - both CT and GC or V CAA - CT only

Methodology:

 Nucleic acid amplification by strand displacement amplification

Performed:

 Clinical Microbiology

Turnaround Time:

 Test is performed daily, Monday through Friday

Specimen Requirements: Patient Preparation: Female - endocervical collection or urine samples; male - urethral sample. Both -  urine samples, throat or rectal

Collect: Follow the directions in the ProbeTec  collection kit.  (Specimen collection kit for the BD ProbeTec Chlamydia trachomatis/Neisseria gonorrhoeae (CT/GC) Qx Amplified DNA Assays).

Collect first morning urine sample in sterile cup or in the transport tube in collection kit. Liquid Pap Collection Devices may also be submitted for this test.

Collect throat swabs using the female (pink) ProbeTec collection kit.  Swab the back of the throat near the tonsils. 

Collect rectal swabs using the female (pink) ProbeTec collection kit.  Insert the swab tip approximately one inch beyond the anal sphincter and rotate.

Specimen Preparation: Urine samples - sterile cup with at least 20 ml of urine and no preservatives.

Storage/Transport: Urine samples and Liquid Pap containers - refrigerator, Probe Tec collection tubes - room temperature

Stability: Urine samples - 24 hours if refrigerated, Liquid Pap containers - 30 days if refrigerated, ProbeTec samples - 30 days room temperature or refrigerated

Causes for Rejection: Failure to use ProbeTec Qx collection kit, samples from non-approved sources.  Urine in tube with preservatives.

Clinical Information:

This test is not recommended for medico-legal purposes (cultures are). Collect C. trachomatis and GC cultures in cases of suspected sexual abuse.

Reference Range:

 

Note:

 Collection kits specific for the ProbeTec system must be used unless liquid Pap sample or urine are collected. No other transport system is acceptable. One transport may be submitted for both Chlamydia trachomatis and Neisseria gonorrhoeae testing. ProbeTec transport for male and female collection available through Materials Management.

CPT 4 Code:

 87491

Review 7/8/2013 by T. Friloux

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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