Laboratory Test Directory

CLOStridium difficile - toxigenic  ASSAY: (088-0511)

Pseudomembranous colitis; detection of toxigenic Clostridium difficile from unformed or liquid fecal samples.

 V CDT

This molecular assay is based on loop-mediated amplification technology. The assay contains primers that specifically amplify a 204 bp region of the conserved 5’ sequence of the tcdA gene within the PaLoc of toxigenic C. difficile. Meridian illumipgene assay.

 Clinical Microbiology

 Test is performed daily

Collect: Feces  - liquid or unformed

Specimen Preparation:  Clean container with no preservative  or Cary-Blair preservative

Storage/Transport: Transport overnight at room temperature or refrigerate samples

Stability: 1 day at room temperature, unpreserved; 5 days, refrigerated; indefinitely, frozen

Causes for Rejection:  Submission of Culturette (swab), leaking container, formed stool, stool in preservatives. Specimen container unlabeled or labeled incorrectly. No date and time of collection on requisition form.

Negative

Test is performed once every 7 days per negative patient and once per hospitalization for positive patient, unless otherwise approved by Clinical Microbiology Director or designee.

 87493

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

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