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CHLAMYDIA TRACHOMATIS (ct) AMPLIFIED ASSAY: (088-0093)
Clinical Indication:

 Detection of Chlamydia trachomatis in urine, endocervical, urethral and vaginal specimens
Test Mnemonic:

 V CAA - Chlamydia alone or V NCAA for both Chlamydia and Gonorrhoeae
Methodology:

 Nucleic acid amplification by strand displacement amplification
Performed:

 Clinical Microbiology
Turnaround Time:

 Daily Monday through Friday
Specimen Requirements:

Patient Preparation: Vaginal samples from women younger than17 years of age or from pregnant women have not been validated and cannot be performed with this assay.

Collect: Follow the collection directions in the ProbeTec kit collection  (Specimen collection kit for the BD ProbeTec Chlamydia trachomatis/Neisseria gonorrhoeae (CT/GC) Qx Amplified DNA Assays).  Collect first catch urine sample in sterile cup or in the transport tube in collection kit. Patient should not have urinated for at least one hour prior to urine collection. Liquid Pap Collection Devices may also be submitted for this test.

Specimen Preparation: Refrigerate urine samples

Storage/Transport: Black top tubes - room temp. Do not allow contact with other patient samples in order to avoid any cross contamination; urine samples - refrigerated

Stability: In collection tubes, samples are stable for 30 days, unpreserved urine samples are stable for 7 days if refrigerated and 30 hours if un-refrigerated.

Causes for Rejection: Failure to use ProbeTec Qx collection kit, samples from non-approved sources such as rectal or eye. Vaginal samples from women younger than 17 or from pregnant women.  Urine in tube with preservatives. Specimen container unlabeled or labeled incorrectly, no date and time of collection on the requisition form, insufficient quantity of urine for test requirements.
Clinical Information:

This test is not recommended for medico-legal purposes (cultures are). Collect C. trachomatis and GC cultures in cases of suspected sexual abuse.

Reference Range:

Negative
Note:

 Collection kits specific for the ProbeTec Q x  system must be used. One transport may be submitted for both Chlamydia trachomatis and Neisseria gonorrhoeae testing. Collection kits are available through Materials Management.
CPT 4 Code:

 87491
Review 2/27/2013 by T. Friloux

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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