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Neisseria gonorrhoeae (gc) amplified assay: (088-0019)
Clinical Indication: Suspected infection with N. gonorrhoeae in genital sources
Test Mnemonic: V NCAA - both GC and Chlamydia trachomatis or V NAA - GC only
Methodology: Nucleic acid amplification by strand displacement amplification
Performed:

 Clinical Microbiology
Turnaround Time: Test is performed daily, Monday through Friday
Specimen Requirements: Patient Preparation: Female - endocervical or vaginal collection; male - urethral sample Both -  urine, throat or rectal samples.

Collect: Follow the directions in the ProbeTec  collection kit (Specimen collection kit for the BD ProbeTec Chlamydia trachomatis/Neisseria gonorrhoeae (CT/GC) Qx Amplified DNA Assays).

Collect first morning urine sample in sterile cup or in the transport tube in collection kit. Patient should not have urinated for at least one hour prior to urine collection. Liquid Pap Collection Devices may also be submitted for this test.

Collect throat swabs using the female (pink) ProbeTec collection kit.  Swab the back of the throat near the tonsils. 

Collect rectal swabs using the female (pink) ProbeTec collection kit.  Insert the swab tip approximately one inch beyond the anal sphincter and rotate.

Specimen Preparation: Urine samples - sterile cup or tube with at least 20 ml of urine and no preservatives.

Storage/Transport: Urine samples and Liquid Pap containers - refrigerator, Probe Tec collection tubes - room temperature. Do not allow contact with other patients' samples in order to avoid any cross contamination.

Stability: Urine samples - 7 days if refrigerated and 30 hours if un-refrigerated, Liquid Pap containers - 30 days if refrigerated, ProbeTec samples - 30 days room temperature or refrigerated

Causes for Rejection: Failure to use ProbeTec Qx collection kit, samples from non-approved sources.  Vaginal samples from women younger than 17 years of age or from pregnant women; urine in tube with preservatives, specimen container unlabeled or labeled incorrectly, no date and time of collection on the requisition form, insufficient quantity of urine for test requirements.  
Clinical Information:

This test is not recommended for medico-legal purposes (cultures are). Collect C. trachomatis and GC cultures in cases of suspected sexual abuse.

Reference Range:

Negative
Note:

Collection kits specific for the ProbeTec system must be used unless Liquid Pap sample or urine are collected. No other transport system is acceptable. One transport may be submitted for both Chlamydia trachomatis and Neisseria gonorrhoeae testing. ProbeTec transport for male and female collection kits are available through Materials Management.
CPT 4 Code:

87591
Review 7/8/2013 by T. Friloux

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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