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HEPATITIS B SURFACE ANTIGEN (088-7200)

Synonyms:  HbsAg, Australian Antigen

CPT 4 Code: 87340

Test Order Mnemonic: HBS

Lab: Serology

Request Form: Microbiology/Serology

Collection: Routine venipuncture, SST (serum separator tube)

Storage Instructions: Centrifuge the specimen and store at 2-8°C

Causes for Rejection: Gross hemolysis, specimens containing particulate matter, heat-inactivated specimens, samples from patients on heparin therapy

Availability: Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough.

Turnaround Time: 24 - 72 hours

Special Instructions: This test is suggested for work-up of acute or chronic infections with hepatitis B virus. If the patient has previously been positive, it is recommended to wait three months before testing again. If the patient has no detectable antigen, it is recommended to wait two weeks before testing again. Tests on samples collected outside of these parameters will be cancelled unless approved by a Microbiology Director or their designee.

Recommended tests to determine acute hepatitis (see Note) are Hepatitis A antibody IgM, Hepatitis B surface antigen, Hepatitis B core antibody IgM, and Hepatitis C antibody.
NOTE: Limited Coverage Test (NCD)

Recommended tests to determine chronic hepatitis are Hepatitis B surface antigen, Hepatitis B core antibody IgM, and Hepatitis C virus antibody.

Specimen: Blood

Volume: 2 ml serum

Container: SST (gel separator)

Reference Ranges: Negative

Test Method: Enchanced chemiluminescence (ECi)

Revised/reviewed 07/25/2008 by T. Friloux

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