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HEPATITIS C VIRUS ANTIBODY (088-7202)

Synonyms: Anti-HCV; Non-A, Non-B Hepatitis

CPT4 Code: 86803

Test Order Mnemonic: HCV

Lab: Serology

Request Form: Microbiology/Serology

Collection: Routine venipuncture, SST (serum separator tube)

Storage Instructions: Centrifuge the specimen and store at 2-8°C.

Causes for Rejection: Gross hemolysis, specimens containing particulate matter, heat-inactivated specimens, submission of samples in tubes other than SST

Availability: Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough

Turnaround Time: 24 - 72 hours

Special Instructions: This test detects both IgG and IgM to HCV. Previously positive patients do not require repeat testing. If the patient has no detectable antibody, it is recommended to wait at least three months before testing again. Tests on samples collected less than three months from the initial negative results will be cancelled, unless approved by a Microbiology Director or designee. Samples with a low level of antibody are confirmed by PCR testing.  Refer to HCV Qualitative PCR.

Recommended tests to determine acute hepatitis (see Note) are Hepatitis A virus antibody IgM, Hepatitis B Surface antigen, Hepatitis B Core antibody IgM and Hepatitis C virus antibody.
NOTE: Limited Coverage Test (NCD)

Recommended tests to determine chronic hepatitis are Hepatitis B Surface antigen, Hepatitis B Core antibody IgM and Hepatitis C virus antibody.

Specimen: Blood

Volume: 2 ml serum

Container: SST (gel barrier)

Reference Ranges: Negative

Method: Enchanced chemiluminescence (ECi)

Revised Feb. 2006 by T. Friloux

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