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influenza A virus, influenza b virus and rsv virus, by pcr: (088-0130)
Clinical Indication:

Differential diagnosis of respiratory disease caused by Influenza A virus, Influenza B virus or Respiratory Syncytial  virus.
Test Mnemonic:

 V PCRABR
Methodology:

 Reverse transcription polymerase chemical reaction
Performed:

 Clinical Microbiology
Turnaround Time:

 1-5 days
Specimen Requirements: Patient Preparation:  Nasopharyngeal swab, nasal swab, throat swab, nasal aspirate, nasal wash

Collect:

Nasopharyngeal swab: insert fine-tip Dacron or flocked swab past nasal turbinates into posterior nasopharynx. Turn swab several times and remove.

Nasal swab: insert Dacron or flocked swab into nostril, turn several times and remove. 

Throat swab: rub tonsilar area of throat with Dacron or flocked swab. 

Nasal aspirate: insert flexible tubing past nasal turbinates into nasopharynx. Attach mucus trap or syringe to other end of tubing and apply suction.  Remove tubing from nose. It may be necessary to draw a small volume (approx 1 ml) of sterile saline through the tubing to collect mucoid secretions into trap or syringe.

Nasal wash: introduce 1-2 ml of sterile saline into the nose using a sterile bulb, and immediately apply suction to collect saline plus secretions.  Alternatively, for older patients, tilt patient’s head back, pour 1-2 ml of sterile saline from a cup into a nostril, while the patient is humming (to avoid swallowing the saline).  Return patient’s head to normal position, while keeping the cup under the nose to collect secretions.

Specimen Preparation: Place swab in Virus transport medium.  Fluids (aspirate or wash) should be collected in a sterile container. 

Storage/Transport: Keep specimens or VTM cold and transport on ice.

Stability: 48 hours on ice

Causes for Rejection:  Inappropriate source.  Prolonged storage at room temperature. Repeat samples. Specimens received in non-sterile containers. Wooden swabs
Clinical Information:

 
Reference Range:

Not detected
Note:

Viral Transport Medium and flocked swabs are available from the Clinical Microbiology lab located in Room 5.137 McCullough.

All three viruses are tested and reported with this test method.

Subtyping for Influenza A available upon approval of Microbiology Lab  Director.
CPT 4 Code:

 87801x3
Review 9/27/2012 by T. Friloux

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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