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quantitative tissue culture:(088-8136)
Clinical Indication:

Surgery: Quantitative cultures yielding greater than ten to the fifth organisms per gram of tissue are associated with unsuccessful skin grafts and delayed wound closures.

Burns: Quantitative cultures yielding greater than ten to the fifth organisms per gram of tissue are associated with invasive burn wound infection and unsuccessful skin grafts.
Test Mnemonic:

 C QUANT
Methodology:

 Culture, numeric quantitation of bacteria and yeast in biopsy samples; gram stain
Performed:

 Clinical Microbiology
Turnaround Time:

 2-5 days
Specimen Requirements: Patient Preparation:

Prep the eschar or skin with Isopropyl alcohol on dampened gauze to remove any topical agent and destroy any surface flora.

Allow the surface to dry.

Tissue quantitative cultures work best with punch biopsies; however, the laboratory will accept biopsies up to 1cm x 1cm and as deep as necessary. (Large tissue samples are a biohazard, as laboratory personnel must use sharps to reduce the size).

Collect:  Biopsy specimens from infected areas

Specimen Preparation:  Place specimen in sterile container. Do not add formalin or saline.

Storage/Transport: Room temperature; transport immediately

Stability: 12-24 hours

Causes for Rejection: Insufficient quantity, non-sterile container, non-graft surgery, mislabeled or insufficiently labeled specimens or request forms.
Clinical Information:

Fastidious organisms requiring special media may not be detected. If unusual organisms are suspected, prior notification of the laboratory is necessary so that an alternate media can be used, or incubation time can be extended.
Reference Range:

No growth
Note:

 Do not add any fluid to the container.

Refer to Tissue culture for routine bacterial culture of biopsy samples.
CPT 4 Code:

  87071: Culture, 87015: Concentration, 87205: Gram Stain
Review 6/10/2013 by T. Friloux

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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