RESPIRATORY SYNCYTIAL VIRUS, DIRECT (088-0074)
CPT4 Code: 87420 (EIA)
Test Order Mnemonic: V RSE
Applies to: The EIA test is an enzyme immunoassay where viral antigens are captured on a membrane and reaction is visible by color change.
Request Form: Microbiology
Collection: Use Sterile saline (PBS) to aspirate a nasal wash. With a rubber bulb, insert 5 ml of PBS into the patient's nostril. Immediately aspirate the PBS back into the bulb. Transfer the contents of the bulb to an empty test tube and label.
Storage Instructions: Refrigerate. Transport on ice.
Causes for Rejection: Specimen not transported on ice, insufficient cellular material, frozen samples
Availability: Samples are accepted 24 hours per day at the Microbiology Processing Laboratory, Room 5.137 McCullough.
Turnaround Time: 1 day; STAT 2 hours
Special Instructions: Transport on ice. Instructions and materials are available from Microbiology Receiving, Room 5.137, McCullough Building, Monday- Sunday, 8 AM- 11: 00 PM.
Other information: The EIA test is available during the viral respiratory season (usually November through April). The RSV PCR test or shell vial culture will be performed on samples with a negative direct result.
Specimen: Nasal wash. nasopharyngeal swab, nasal aspirate, BAL Nasal wash and NP swab/aspirate are most appropriate since this is where the highest titers of virus are contained.
Minimum Volume: 3 ml
Container: Test tube with screw cap
Test Method: EIA membrane assay.
Revised 10/21/2011 by T. Friloux