Laboratory Test Directory

Test Order Mnemonic:  Syphilis Testing: SYPG

Test Includes: All samples are screened with the Syphilis IgG  test. If the screening test is positive or indeterminate, RPR titer and confirmation test (TP-PA) are performed. RPR titer is performed on previously positive positive patients samples, but confirmation is not required.

Lab: Serology

Request Form: Microbiology/Serology

Collection: Routine venipuncture, SST (serum separator tube)

Storage Instructions: Centrifuge and store at 2-8°C.

Causes for Rejection: Hemolysis, submission of tubes other than SST

Availability: Samples are accepted 24 hours per day at Sample Management, 5.136 McCullough.

Special Instructions: Guidelines for testing patient samples:  For patients diagnosed with syphilis (i.e., positive IgG and confirmatory test), the CDC recommends that response to therapy be assessed by testing the RPR (looking for a 4-fold decline in titer) after 6 months. Exception: Specimens from Labor and Delivery are tested if seven days have elapsed since the last test. Tests are performed on all obstetrics patients regardless of previous results.  If there is no previous patient data, the requested tests are performed.

Specimen: Blood

Volume: 2ml serum

Container: SST (gel separator); red top - remove serum from clot before submission unless immediate transport is possible

Reference Ranges: Non-reactive