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BRAF mutation DETECTION: (138-0022)
Clinical Indication:

 Detection of BRAF Codon 600 Mutation
Test Mnemonic:

 BRAF
Methodology:

 PCR, pyro sequencing
Performed:

 Molecular Diagnostics Laboratory
Turnaround Time:

 7-14 days
Specimen Requirements: Patient Preparation: Tumor sample

Collect: Formalin fixed (10 percent neutral buffered formalin) and paraffin embedded tissue sections (prepared in Surgical Pathology).  Transport 5 unstained 5-10 micron slides (Minimum is 3 slides) with a matching HE slide marked tumor area. Tumor cell smear/cell block (prepared in Cytopathology) or blood samples may be used when there are enough tumor cells in the sample.

Specimen Preparation: Slides are prepared by Surgical Pathology.

Storage/Transport: Protect specimens from excessive heat. Ship in cooled container during summer months. Transport are room temperature or refrigerated.

Causes for Rejection: Specimens with less than 10% tumor cells. Incomplete and/or incorrect specimen identification.
Clinical Information:

May serve as predictive marker for anti-EGFR treatment - reflex when KRAS is negative; may serve as predicative marker for anti-mutant BRAF treatment, for tumor classification and prognosis
Reference Range:

Not detected
Note:

 
CPT 4 Code:

 81210 and 88381
Review 1/31/13by T. Friloux and Dr. Dong

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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