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Cytomegalovirus (CMV) dna, real-time pcr assay: (138-7497)

Clinical Indication:

 To detect and monitor active CMV infections

Test Mnemonic:

 CMV PCR

Methodology:

 Real-time PCR

Performed:

 Molecular Diagnostics Laboratory

Turnaround Time:

 3-7 days, please call lab at (409)772-4197 if quicker turnaround time is needed

Specimen Requirements: Patient Preparation: Various samples are accepted for testing, including  plasma, serum, whole blood, urine, CSF, BAL, biopsy tissue, etc.

Collect: Blood - PPT (white top) or gold (SST) tube. Urine and other sample types, collect in a sterile container.

Specimen Preparation: Within four hours of collection, centrifuge PPT tube at 1000g for 15 minutes.  Do not centrifuge whole blood or bone marrow samples. No specimen prep required for urine or other sample types.

Storage/Transport: Room temperature. Store refrigerated or frozen if delay in transport.

Stability: 24 hours

Causes for Rejection: Incomplete and/or incorrect sample identification, insufficient sample collection, gross hemolysis, clotted samples, incorrect tube type, non-sterile or leaking containers

Clinical Information:

 

Reference Range:

None detected; <200 copies/mL

Note:

 Submit one PPT tube for each molecular assay requested due to volume needs for testing.

This assay measures cytomegalovirus (CMV) DNA using real-time PCR. The dynamic range of the assay is 200-100,000,000 copies per ml.

A result of <200 copies/mL does not rule out the presence of PCR inhibitors in the patient's specimen or that CMV virus DNA concentrations may be below the level of quantification. Care should be taken when interpreting any single viral load determination and the test is intended for use in conjunction with clinical presentation and other laboratory results.

CPT 4 Code:

 87497

Review T. Friloux and Dr. Dong 1/15/2013

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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