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T CELL MONOCLONALITY BY PCR (138-0072) Synomons: Gene rearrangement (T-cell), Lymphoma (T cell) by PCR CPT 4 CODE: 83890, 83894, 83897, 83898, 83903 Test Order Mnemonic: CT PCR Applies to: Presence of gamma T-cell gene rearrangement Lab: Molecular Diagnostic Laboratory Request Form: Must be manually written on any of the available Laboratory Request Forms with signature approval from Director of Laboratory or Hematopathologist Collection: Routine venipuncture, tissue biopsy, bone marrow aspiration, paraffin embedded tissue Storage Instructions: Refrigerate whole blood, bone marrow aspirate, store fresh tissue at -70° C Causes for Rejection: Serum, heparin collection tube Availability: Samples are accepted Monday –Friday, 8:00 AM to 5:00 PM, Room 5.212, John Sealy Annex with consultation and approval by Director or Hematopathologist Special Instructions: Do not Centrifuge or separate specimen. B5 fixed paraffin embedded tissue is not acceptable. Specimen: Whole blood with EDTA or Sodium Citrate, Fresh frozen tissue, Bone Marrow Aspirate, Paraffin embedded tissue Volume: 5 mls. whole blood or aspirate, 0.1 grams tissue Container: EDTA (purple) or sodium citrate (blue), or sterile container for tissue Reviewed by Jianli Dong, MD, PhD 03/2006
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