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hepatitis b virus dna real-time pcr: (138-0476)
Clinical Indication:

Detection and quantification of HBV DNA
Test Mnemonic:

 HBV PCR
Methodology:

 Real time PCR
Performed:

 Molecular Diagnostics Laboratory
Turnaround Time:

 3-10 days
Specimen Requirements: Patient Preparation: Routine venipuncture

Collect: One pearl color  (PPT) top Vacutainer; collect one tube for this tests and do not combine with other test orders.

Specimen Preparation: Sample must be processed within 6 hours of collection for optimal recovery of DNA. Plasma: separate from cells by centrifugation at 1,000 x g for 15 minutes. Separate and store frozen, after centrifugation.

Storage/Transport: Transport at room temperature or refrigerated temperature.

Stability: Separation must occur within 6 hours of collection, store frozen for up to  one month.

Causes for Rejection: Incomplete and/or incorrect sample identification, insufficient sample volume, incorrect blood collection tube (Vacutainer), gross hemolysis, clots, prolonged time to processing
Clinical Information:

Patient has chronic Hepatitis B virus infection and it is being treated. This is not a test to use to diagnose Hepatitis.
Reference Range:

Interpretation of Results:

Not detected: HBV not detected

<10 IU/mL: HBV detected but <10 IU/mL

10-109IU/mL: Quantity of virus detected will be reported

>109 IU/mL: Viral load above the upper limit of quantification.
Note:

 Submit one PPT tube for each molecular assay requested due to volume needs for testing.

The assay uses the FDA approved Abbott RealTime HBV kit and m2000 system .

A "not detected" result does not rule out the presence of HBV in the patient's specimen or that HBV DNA concentrations may be below the level of quantification.
CPT 4 Code:

 87517
Review 1/15/2013 T. Friloux and Dr. Dong

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient.  Components of the organ or disease panels may be ordered individually.  The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-9 code or its verbal equivalent.

 

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