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T-CELL GENE REARRANGEMENT (138-3912) CPT 4 CODE: 83891, 83892, 83894, 83896, 83897 Test Order Mnemonic: T-CELL Applies to: Presence of beta T-cell gene rearrangement Lab: Molecular Diagnostic Laboratory Request Form: Must be manually written on any of the available Laboratory Request Forms with signature approval of Director of Laboratory Collection: Routine Venipuncture, tissue biopsy, bone marrow aspiration Storage Instructions: Refrigerate whole blood, bone marrow aspirate, store fresh tissue at -70° C Causes for Rejection: Serum, heparin collection tube Availability: Samples are accepted Monday-Friday, 8:00 AM to 5:00 PM, Room 5.212, John Sealy Annex with consultation and approval from Director or Hematopathologist Special Instructions: Do not Centrifuge or separate specimen. CT PCR will be performed initially. A negative result will be evaluated by Director or Hematopathologist for appropriateness of gene rearrangement being performed. Specimen: Whole blood with EDTA or Sodium Citrate, Fresh frozen tissue, Bone Marrow Aspirate Volume: 10 mls. whole blood or aspirate, 0.5 grams tissue Container: EDTA (purple) or sodium citrate (blue), or sterile container for tissue Reviewed by Jianli Dong, MD, PhD 03/2006
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