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Urine Specimen Collection
           
Random Urine Collection
First voided morning specimens should be collected in clean
containers and transported to the laboratory within 30 minutes or stored according to the
specific instructions
Timed Urine Collection
To ensure accurate results, it is essential that an
accurate collection of the timed output of urine is achieved. Follow these
guidelines to collect a timed urine:
- At the beginning of the collection period, the
bladder should be emptied.
- Discard this sample and note the time on the
requisition form.
- Collect all urine passed from this point on.
- Specimens are placed in the timed urine bottle which
may contain preservatives. Use caution when adding the urine to bottles containing
acid preservatives. For safety purposes, instruct the patient to urinate in a clean,
dry container, then transfer the urine to the collection bottle. The patient should
not urinate into the collection bottle.
- If one of the samples is
accidentally lost or removed within the collection period, the entire collection must be
restarted.
- At the end of the collection period the bladder is
emptied and the specimen is added to the collection.
- Note the ending time on the request form.
- If removing an aliquot of urine, the total volume
for the timed collection must be noted on the request form.
Clean Catch, Mid-Stream Urine collection:
Females
- Remove appropriate garments for urination.
- Wash hands thoroughly.
- With one hand, separate the urinary opening and hold
open until the process is complete.
- Using a saturated soapy sponge, wash from front to
back three times using a different soap and pad each time.
- Wash with warm sterile water to remove soap residue.
- The patient should void a few milliliters into the
toilet and then collect urine in a sterile container. (Do not stop the flow of urine
through this process.)
- Secure the lid on the container.
- The nurse will transfer the specimen for culture to
the gray-topped vacutainer tube through needle apparatus on urine cup. Refer to
package insert for further information/instructions.
Males
- Remove appropriate garments for urination
- Wash hands thoroughly with soap and water.
- Cleanse the penis, retract the foreskin (if not
circumcised), and wash three times with a different soap pad each time.
- Wash with warm sterile water to remove the soap
residue.
- Keeping the foreskin retracted, allow a few
milliliters of urine to pass and then collect urine in a sterile container. (Do not stop
the flow of urine through this process.
- Secure the lid on the container.
- The nurse will transfer the specimen for culture to
the gray-topped vacutainer tube through needle apparatus on urine cup. Refer to
package insert for further information/instructions.
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