Our work focuses on elucidating the molecular mechanisms of G protein-coupled receptors. These are seven-helix transmembrane receptors acting as detectors of signals including odor, light, hormones, neurotransmitters, and as mediators for the entry of HIV-1. Genes encoding these receptors occupy a hefty 3% of the human genome. Of importance is that these receptors are the target of about 60% of drugs used to treat human diseases. In my laboratory we are applying X-ray crystallography to elucidate the atomic structure of seven-helix transmembrane receptors. Genetic and biophysical approaches are also applied to determine the role of specific domains on the function of these receptors. We are also investigating the molecular mechanisms underlying the chemokines-regulated the inflammation-neurogenesis coupling.
Yasser Qutub, Ilya Reviakine, Carrie Maxwell, Javier Navarro, Ehud Landau, Peter G. Vekilov. In cubo mechanisms of growth and defect formation of three-dimensional bacteriorhodopsin crystals. J. Mol.Biol 343:1243-1254, 2004
Sarmiento, J., Añazco C.C.,Campos, D., Prado, L., Vergara, L., Navarro, J. and Gonzalez, C. Regulation of the Surface Expression of the Vasopressin Receptor V2 by Dimerization with an Alternative Splice Variant. J.Biol.Chem. 279: 47017-47023, 2004.
Sutton, R.B., Vishnivetskiy, S.A., Robert, J., Hanson, S.M., Raman,D., Knox B.E., Kono, M., Navarro, J., Gurevich, V.V. Crystal Structure of Cone Arrestin: Evolution of Receptor Specificity. J Mol Biol. 16:1069-1080, 2005.
Rajarathnam, K., Prado, G.N., Fernando, H., Clark-Lewis, I., and Navarro, J. Probing Receptor Binding Activity of CXCL8 Dimer Using a disulfide 'trap' Biochemistry 45:7882-7888, 2006
Prado GN, Suetomi K, Shumate D, Maxwell C, Ravindran A, Rajarathnam K, Navarro J., Chemokine Signaling Specificity: Essential Role for the N-Terminal Domain of Chemokine Receptors.Biochemistry 46:8961-8968, 2007
Lida Q. Fuentes, Carlos E. Reyes, José M. Sarmiento, Carolina I. Villanueva, Carlos D. Figueroa, Javier Navarro, Carlos B. González. Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways Cellular Signalling,In Press, Accepted Manuscript, Available online 25 May 2008