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Professor of Neurology
Undergraduate education in biology and physics,
1963 Graduate education in Biophysics (1965) and Biochemistry (1966)
Graduate education in Biochemistry, Ph.D. (1982)
Beijing (Peking) University, Beijing, China Postdoctoral Research in
Neurochemistry in Department of Neurology, Stout Neuroscience Mass
Spectrometry Laboratory, University of Tennessee at Memphis, College
of Medicine, 1986-1987. Sponsor: Dr. D. M. Desiderio.
To identify substances that contribute to
secondary cell death and to explore the molecular mechanisms of
neuron degeneration and death caused by these substances in order to
develop therapeutic strategies to prevent such death following
central nervous system injury and neurological disorders. The
currently research project is to investigate the roles and pathways
by which reactive species cause cell death, particularly apoptosis,
after spinal cord injury and to explore the therapeutic potential of
a metalloporphyrin - a superoxide dismutase mimetic and a broad
spectrum scavenger of reactive species - in spinal cord injury and
other neurological disorders.
To identify the proteins most susceptible to
oxidative damage and the proteins important in apoptotic cell death
pathways following central nervous system injury and neurological
disorders by utilizing proteomic technology. this will offer
accurate targets for therapeutic intervention.
Research Support: The research projects
were continuously supported by the PI's grants from NIH (NINDS,
RO1s) and other agencies.
Teaching Responsibility: Lectures in
graduate and medical school courses and directs a course "Proteomics
in Neuroscience: in the graduate school.
Grant Review for the NIH (study section:
Special Emphasis Panel for RFA in 2000), Paralyzed Veterans of
America, Spinal Cord Research Foundation, Department of Veterans
Affairs, Western Research & Development Center
Journal Reviewer For Annuals of Neurology, the
Journal of Neuroscience, Journal of Neurochemistry, Neuroscience,
Experimental Neurology, Brain Research, Neuroscience Letters, Free
Radical Biology and Medicine, Neurochemistry International,
Anesthesiology
1. Liu, D., Bao, F., Prough, D. S., and Dewitt, D.
S. Peroxynitrite generated at the level produced by spinal cord
injury induces peroxidation of membrane phospholipids in normal rat
cord: Reduction by a metalloporphyrin. J. Neurotrauma,
22: 1123-1133, 2005.
2.
Bao, F., and
Liu, D., Hydroxyl radicals generated
in the rat spinal cord at the level produced by impact injury induce
cell death by necrosis and apoptosis: Protection by a
metalloporphyrin. Neuroscience, 126: 285-295, 2004.
3. Liu, D., Liu, J., sun, D., and Wen, J. The time
course of hydroxyl radical formation following spinal cord injury:
the possible role of the iron-catalyzed Haber-Weiss reaction. J. Neurotrauma, 21: 805-816, 2004.
4.
Bao, F., and
Liu, D., Peroxynitrite generated in
the rat spinal cord induces apoptotic cell death and activates
caspase-3. Neuroscience, 116: 59-70, 2003.
5.
Bao, F., Dewitt, d. S., Prough, D. S., and
Liu, D., Peroxynitrite generated in the rat spinal cord induces oxidation
and nitration of proteins: Reduction by Mn (III) tetrakis
(4-benxoic acid) porphyrin. J. Neurosci. Res., 71:
220-227, 2003.
6. Liu, D., Liu, J., Sun, D., Alcock, N. W., and
Wen, J. Spinal injury increases iron: Catalytic production of
hydroxyl radicals. Free Rad. Biol. Med., 34: 64-71,
2003.
7.
Bao, F., and
Liu, D., Peroxynitrite generated in
the rat spinal cord induces neuron death and neurological deficits.
Neuroscience, 115: 839-849, 2002.
8. Liu, D., Li, L., and Augustus, L.
Prostaglandin release by impact injury mediates hydroxyl radical and
malondialdehyde production and cell death: A site of the
neuroprotective action of methylprednisolone. J. Neurochem.
77: 1036-1047, 2001.
9.
Leski, M. L., Bao, F., Wu, L., Qian, H., Sun, D.,
and
Liu, D., Protein and DNA oxidation in spinal injury:
Neurofilaments - an oxidation target. Free Rad. Biol. Med.,
30: 613-624, 2001.
10. Liu, D., Ling, X., Wen, J., and Liu, J. The
role of reactive nitrogen species in secondary spinal cord injury:
Formation of nitric oxide, peroxynitrite, and nitrated protein. J. Neurochem. 75: 2144-2154, 2000.
11.
Liu, D.,
Wen, J., Liu, J., and Li, L. The
roles of free radicals in amyotrophic lateral sclerosis:
Reactive oxygen species and elevated oxidation of protein, DNA and
membrane phospholipids. FASEB Journal, 13: 2318-2328,
1999.
(Accepted without revision)
12.
Liu, D., Liu, J., and Wen, J. Elevation of
hydrogen peroxide after spinal cord injury detected by using the
Fenton reaction. Free Rad. Biol. Med. 27:
478-482, 1999.
13.
Liu, D., Sybert, T. E., Qian, H., and Liu, J.
Superoxide production after spinal injury detected by microperfusion
of cytochrome c. Free Rad. Biol. Med. 25: 298-304, 1998.
14.
Qian, H., and
Liu, D., The time course of
malondialdehyde production following impact injury to rat spinal
cord as measured by microdialysis and high pressure liquid
chromatography. Neurochem. Res. 22:
1231-1236, 1997.
Liu, D.,
Yang, R., Yan, X., and McAdoo, D.J. Hydroxyl radical generated
in vivo kills neurons in spinal cord: electrophysiological, histological,
and neurochemical results. J. Neurochem. 62: 37-44, 1994.
(Accepted without revision)
Liu, D., Sybert, T.E., Qian, H., and Liu, J. Superoxide
production after spinal injury detected by microperfusion of
cytochrome c. Free Rad. Biol. Med. 25: 298-304, 1998.
Liu, D., Wen, J., Liu, J., and Li, L. The roles
of free radicals in amyotrophic lateral sclerosis: Reactive
oxygen species and elevated oxidation of protein, DNA and membrane
phospholipids. FASEB Journal 13: 2318-2328, 1999.
Liu, D., Liu, J., and Wen, J. Elevation of hydrogen peroxide
after spinal cord injury detected by using the Fenton reaction. Free
Rad. Biol. Med. 27: 478-482, 1999. (Accepted without
revision)
Liu, D., Ling, X., Wen, J., and Liu, J. The role of reactive
nitrogen species in secondary spinal cord injury: Formation
of nitric oxide, peroxynitrite, and nitrated protein. J.
Neurochem. 75: 2144-2154, 2000.
Liu, D., Li, L., and Augustus, L. Prostaglandin release
by impact injury mediates hydroxyl radical and malondialdehyde
production and cell death: A site of the neuroprotective action
of methylprednisolone. J. Neurochem. 77: 1036-1047,
2001.
Leski,
M.L., Bao, F., Wu, L., Qian, H., Sun, D., and
Liu, D.,. Protein
and DNA oxidation in spinal injury: Neurofilaments - an oxidation
target. Free Rad. Biol. Med, 30: 613-624, 2001.
Bao,
F., and
Liu, D., Peroxynitrite generated
in the rat spinal cord induces neuron death and neurological
deficits. Neurosci., 115: 839-849, 2002.
Bao,
F., and
Liu, D., Peroxynitrite generated
in the rat spinal cord induces apoptotic cell death and activates
caspase-3. Neurosci., 116: 59-70, 2003.
Liu, D., Liu, J., Sun, D., Alcock, N. W., and Wen, J. Spinal
injury increases iron: Catalytic production of hydroxyl
radicals. Free Rad. Biol. Med., 34: 64-71, 2003. |
