 |
|
Synthetic Organic Chemistry Core
Core Director and Members
The Synthetic Organic Chemistry Core (SOC) was first established within the Sealy Center for Molecular Science and is an integral part of the NIEHS Center at UTMB and now part of the Sealy Center of Environmental Health and Medicine. The SOC: 1) provides an on-campus cost-efficient chemical synthesis facility with expertise in synthesis of defined chemical DNA lesions, bioconjugates and small organic molecules for environmental, biological and medical research which are unavailable elsewhere or prohibitively expensive commercially, and 2) to reduce costs for this service by sharing this resource campus wide and externally among several academic research groups. The SOC program for achieving these goals utilizes known literature methods and develops new methods and protocols when necessary. The SOC specifically has expertise in the synthesis of modified nucleosides representing known site-specific DNA damage and also development of methods and protocols for purifying and analyzing these defined products.
The Director of the SOC is Dr. Richard P. Hodge, Ph.D., a Scientist in the Sealy Center for Environmental Health and Medicine. He and the other members of the SOC, Ms. Linda C. Hackfeld, M.S., (Research Associate III) and Ms. Stacia Crocker (Research Associate I), provide scientific expertise and technical support for the NIEHS center Investigators in conducting a wide range of organic chemistries, purification methods and analyses. The SOC is housed in Room 2.205 of the Basic Science Bldg., adjacent to the Truman Blocker Medical Research Bldg. and the NIEHS Center at UTMB. The SOC is currently a 700-sq. ft. full service synthetic organic chemical facility utilizing state of the art equipment capable of carrying out multistep organic syntheses from mg to 100g scales. Capabilities include reactions up to 2L volumes, temperature controlled reactions from -78oC to 200oC, photochemical reactions, rotary evaporation and lyophilizations up to 5L, flash and gravity feed chromatography of up to 3Kg of media, oligonucleotide syntheses from 20nM to 30uM scale, Analytical and Preparative HPLC and Karl-Fischer water titrations.
The SOC has since inception of the NIEHS Center, provided an invaluable synthesis service to Center investigators, and continues to meet new demands as they arise. In the past funding year, (abbreviated 7 weeks due to Hurricane Ike), the SOC provided services to 12 NIEHS Center Investigators (Table C). Synthesis of small molecules and bioconjugates continues to represent our primary activity however there was an increase in requests for modified (UV and alkylation damaged) oligonucleotide synthesis services for investigators in the DNA Repair & Mutagenesis Research Core, primarily from the Prakash group, and also included one Pilot project (EDTA modified oligonucleotide) for Dr. Iwahara. The Biotransformation Research Core investigators who sought our services (primarily small molecule syntheses) included Drs. N. Ansari and S. Ansari, Halpert, Elferink and Pikuleva. The SOC also performed synthesis of molecular weight standards for mass spectrometry for Dr. Kurosky’s group in the Biomolecular Research facility core. Four other non-NIEHS Center UTMB investigators also used the facility for synthesis of small molecules or research support. External users included UV modified oligos for the Univ. of Alberta, Canada and a Cs124 chelator-linker for labeling proteins for Texas Tech University in Lubbock, TX. The immediate benefit of the SOC to Center Investigators is that the Center grant pays one-half the reagent costs associated with any service provided by the SOC to Center members. The core enhances the Center by providing access to and expertise in organic synthesis as well as operation and maintenance of equipment that would be prohibitively expensive to Center members if duplicated in individual Center Investigator's laboratories.
Core Director and Members
Richard P. Hodge, Ph.D., Director,
Synthetic Organic Chemistry Service Core, Scientist, Sealy Center for
Environmental Health & Medicine
Linda C. Hackfeld, M.S., Member, Synthetic Organic Chemistry
Service Core, Research Associate III, Sealy Center for
Environmental Health & Medicine
Stacia
Crocker,
Member, Synthetic Organic Chemistry Service Core, Research Associate I,
Sealy Center for Environmental Health & Medicine
Important Links
SOC
http://research.utmb.edu/starline/research/resres/synthetici.htm
SOC
http://www.scms.utmb.edu/cores/soccl/index.htm
SOC Forms & Pricing:
|
