Prairie Technologies Ultimas IV, multiphoton microscope for in vivo imaging of infectious samples at BSL3 containment (GNL):
This system is a new addition to the core, installed within the GNL-BSL3 containment laboratory is a platform specially designed to perform intravital imaging in small animals but can also be used to image tissue slices and other smaller samples. The microscope is installed inside a class I biosafety cabinet to ensure maximum protection to the operator and environment. For multiphoton imaging this system is equipped with an Spectra Physics Maitai-DeepSee ultrafast tunable laser and two external non-descanned detectors. For high resolution imaging this system is equipped with high numerical aperture water immersion objectives specially designed for multiphoton microscopy. This system is dedicated for the study of live cell preparations infected with agents requiring BSL2 or BSL3 containment. Access is restricted to authorized users of the GNL-BSL3 bio-containment facility. This microscope is not available for independent operation, a member of the core staff should be running the system operation at all times during the imaging sessions.