Pathology Microscope Imaging Laboratory

The Pathology Imaging Core Laboratory is located on the 2nd floor of the Keiller Building, Room 2.142.

The Pathology Imaging Core Lab Director is Gustavo Valbuena, M.D., Ph.D.

This is a BSL-1 facility equipped with three modern Olympus microscopes:

Olympus Is71 Manual Inverted Microscope

Olympus BX61 Fully Motorized Fluorescence Microscope
(http://www.olympusamerica.com/seg_section/product.asp?product=1028)

Olympus DSU-IX81 Spinning Disk Confocal Microscope
(http://www.olympusamerica.com/seg_section/product.asp?product=1009)



Access Procedure

1. View the Training Video(s) for the correct operation of each microscope you are interested in using.
2. Read the required documents and, if possible, the suggested readings found near the end of this page.
3. Complete the appropriate online user agreements below for each microscope you will use and submit form by email to Dr. Gustavo Valbuena (gvalbuen@utmb.edu).
4. Schedule a first visit to the Imaging Facility with Dr. Valbuena in order to assign a user and password for each computer controlling the respective microscopes.
5. Dr. Valbuena will request your card-coded access to the facility and share the Outlook Calendar for scheduling microscope use time.




User Agreements


Please select from the Pathology Microscope Imaging Laboratory PDF documents listed below.

PDF icon, click for the Pathology Education Project Proposal Form
 

Olympus IX71 Manual Inverted Fluorescence Microscope

This is the User Agreement for the Olympus IX71 Manual Inverted Fluorescence Microscope. The form is an interactive PDF document which may be completed and submitted online. Once completed, forward the agreement by clicking the "Submit by Email" button.



PDF icon, click for the Pathology Education Project Proposal Form
 

Olympus BX61 Fully Motorized Upright Fluorescence Microscope

This is the User Agreement for the Olympus BX61 Fully Motorized Fluorescence Microscope. The form is an interactive PDF document which may be completed and submitted online. Once completed, forward the agreement by clicking the "Submit by Email" button.



PDF icon, click for the Pathology Education Project Proposal Form
 

Olympus DSU-IX81 Spinning Disk Confocal Microscope

This is the User Agreement for the Olympus DSU-IX81 Spinning Disk Confocal Microscope. The form is an interactive PDF document which may be completed and submitted online. Once completed, forward the agreement by clicking the "Submit by Email" button.




Training Videos

The Department of Pathology assumes responsibility for assuring that all pathologists, scientists and other personnel who utilize the Pathology Imaging Core facilities are provided with appropriate training and/or instruction. As such, a set of Training Videos has been developed to serve as part of the fundamental instruction for the lab. All new users for the Imaging Core must review the video set below before being granted a user account for the equipment.

Olympus IX71 Inverted Fluorescence Microscope


 

Olympus BX61 Fully Motorized Upright Fluorescence Microscope

How to start the microscope 



How to use manual controls



How to take a DIC image 



How to use fluorescence


How to export images to 8 bit TIFF



How to clean the objectives 


Required Readings

Please read the select PDF documents listed below.


PDF icon, click for the Pathology Education Project Proposal Form
 


Microscope Objectives
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 


Contrast Enhancement in Light Microscopy
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 


Fluorescence Microscopy: A concise guide
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 


Microscope alignment
 

Suggested Readings

The following PDF documents listed below have been suggested for users of the Pathology Microscope Imaging Laboratory.

 

PDF icon, click for the Pathology Education Project Proposal Form
 


Light Microscopy Digital Imaging
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 

Accuracy and precision in quantitative fluorescence microscopy
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 


Basic confocal microscopy
 

 

PDF icon, click for the Pathology Education Project Proposal Form
 

Fluorescence microscopy: Established and emerging methods
 

Links

Relevant links related to microscopy may be found at the website of the Microscopy Society of America

http://www.microscopy.org/resources/links.cfm