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Dr. Kaphalia

Bhupendra Singh Kaphalia, Ph.D.

Professor, Department of Pathology;
Director, Analytical Chemistry Core

University of Texas Medical Branch
301 University Boulevard
Galveston, TX 77555-0438

Office: (409) 772-4995
Fax: (409) 747-1763
bkaphali@utmb.edu

Appointments

2006–Present Director Analytical Chemistry Core, Sealy Center for Environmental Health & Medicine, University of Texas Medical Branch, Galveston, TX
2002–Present Associate Professor Department of Pathology, University of Texas Medical Branch, Galveston, TX

Professional Education

Degree Institution Field of Study Graduation Year
B.S. Agra University, Agra, India Zoology, Botany, Chemistry 1973
M.S. Kumaun University, Nainital, India Chemistry 1975
Ph.D. Kumaun University, Nainital, India Chemistry (Toxicology) 1984

Honors

Editorial board member:

  • Alcohol (Decision Editor)
  • Journal of Toxicology and Environmental Health B.
  • Journal of Experimental Zoology, India
  • The Open Drug Metabolism Journal
  • Toxicology Letters 2002–2005

Reviewer of Peer-Reviewed Journal:

  • Alcohol
  • Bulletin of Environmental Contamination and Toxicology
  • Cell Biology and Toxicology
  • Clinica Chimica Acta
  • Clinical Chemistry and Enzymology
  • Comparative Biochemistry and Physiology
  • Drug Metabolism & Disposition
  • Eastern Research Group, Inc., under contract to USEPA
  • International Journal of Medical Sciences
  • Journal of Experimental Zoology
  • Journal of Toxicology and Environmental Health
  • Lipids
  • Molecular and Cellular Biochemistry
  • Psychiatry Research
  • Toxicology Letters
  • Toxicology and Applied Pharmacology
  • Toxicology Mechanisms and Methods

NIH Study Section Member

Invited Speaker and Reviewer:

  • Symposia speaker
  • Reviewer for ATSDR/EPA Toxicological Profiles/Monographs
  • Chaired and/or co-chaired several scientific sessions

Professional Affiliations

  • Life Member of the Academy of Environmental Biology, India
  • Life Member of the Association of Scientists of Indian Origin in America
  • Member of Society of Toxicology
  • Member of American Chemical Society
  • Member of International Society for the Study of Xenobiotics (ISSX)
  • Member of International Society for Biomedical Research on Alcohol
  • Member of Metabolomic Society
  • Associate member of UTMB’s Experimental Pathology Graduate program
  • Member of UTMB’s Biotransformation Core, NIEHS Center
  • Member of UTMB’s Center for Addiction Research

Research Interests

Objectives: To understand the mechanisms of target organ toxicity of alcohol via lipid conjugation, hypomethylation and biomolecular modifications, and elucidation of the mechanisms involved in alcohol-induced target organ diseases.

Approach: In general, I am working on formation, distribution, metabolism, disposition and toxicity evaluation of lipid conjugates of xenobiotic alcohols and develop analytical methods (HPLC, GC & TLC) involved in in vitro and in vivo metabolism of compounds of biological and environmental interest. I use state of art nuclear magnetic resonance spectroscopy and mass spectrometry for characterization of the metabolites.  Currently, I am working Mechanism-based toxicity studies of ethanol and its metabolites to understand the mechanism(s) of alcoholic pancreatitis.

Selected Publications

  1. Kaphalia BS and Ansari, GAS.  Covalent modification of hepatic microsomal lipids of rats by carbon tetrachloride.  Mol Toxicol 2: 199-213; 1989.
  2. Kaphalia BS.  Fatty acid conjugates of chlorinated phenols:  Synthesis, structural elucidation, and high performance liquid chromatographic analysis.  J Chromatogr 537: 85-92; 1991.
  3. Zeng FY, Kaphalia BS, Ansari GAS, and Weigel PH.  Fatty acylation of the rat asialoglycoprotein receptor: The three subunits from active receptors contain covalently bound palmitate and stearate.  J Biol Chem 270: 21382-21387; 1995.
  4. Kapahlia BS, Carr JB, and Ansari GAS.  Increased endobiotic fatty acid methyl esters following exposure to methanol.  Fundam Appl Toxicol 28: 264-273, 1995.
  5. Kaphalia BS, Fritz RR and Ansari GAS.  Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (pl 6.1).  Chem Res Toxicol 10:211-218, 1997.
  6. Hadjipavlou AG, Simmons JW, Young JP, Ansari GAS, Kaphalia BS, Simmons DJ, Nicodemus CL, Lane R, and Esch O.  Torsional injury resulting in disc degeneration: an in vivo rabbit model.  J Spinal Disorder 11: 312-317, 1998.
  7. Kaphalia BS, Green SG, and Ansari GAS.  Fatty acid ethyl and methyl ester synthases, and fatty acid anilide synthase in HepG2 and AR42J cells: interrelationships and inhibition by tri-o-tolyl phosphate. Toxicol Appl Pharmacol 159: 134-141, 1999.
  8. Kaphalia BS, and Ansari GAS.  Purification and characterization of rat hepatic microsomal low molecular weight ethyl ester synthase and their relationship to carboxylesterases. J Biochem Mol Toxicol. 15: 165-171, 2001.
  9. Kaphalia BS, and Ansari GAS.  Fatty acid ethyl esters and ethanol-induced pancreatitis. Cell. Mol. Biol. 47: OL173-OL179, 2001.
  10. Kaphalia BS, and Ansari GAS.   Purification and characterization of rat pancreatic fatty acid ethyl ester synthase and its structural and functional relationship to pancreatic cholesterol esterase. J Biochem Mol Toxicol. 17; 338-245, 2003.
  11. Kaphalia BS, Ping Cai, M. Firoze, Khan, Okorodudu, A.O. and Ansari GAS. Fatty acid ethyl esters in alcoholics; markers of alcohol abuse and alcoholism. Alcohol 34, 151-158, 2004.
  12. Kaphalia BS, Mericle KA, and Ansari GAS. Mechanism of Differential Inhibition of Hepatic and Pancreatic Fatty Acid Ethyl Ester Synthase by Inhibitors of Serine-Esterases:  In Vitro and Cell Culture Studies. Toxicol. Appl. Pharmacol. 200, 7-15, 2004.
  13. Wu H, Cai P, Clemens DL, Jerrells TR, Ansari, GAS, Kaphalia BS.  Metabolic basis of ethanol-induced cytotoxicity in stable and recombinant HepG2 cells: Role of nonoxidative metabolism. Toxicol. Appl. Pharmacol. 216, 238-247, 2006.
  14. Ping Cai, Konig R, Khan MF, Qiu S, Kaphalia, BS, and Ansari, GAS.  Autoimmune response in MRL +/+ mice following treatment with dichloroacetyl chloride or dichloroacetic anhydride. Toxicol. Appl. Pharmacol. 216, 248-255, 2006.
  15. Bhopale K, Wu H, Boor PJ, Popav, VL, Ansari GAS, and Kaphalia BS.  Metabolic basis of ethanol-induced hepatic and pancreatic toxicity in hepatic alcohol dehydrogenase-deficient deer mice. Alcohol 39, 179-188, 2006.
  16. Kaphalia L, Kaphalia BS, Kanz MF and Moslen MT. Efficient high pressure liquid chromatograph/UVmethod for detection of Diclofenac and 4¢-OH-diclofenac in serum.  J Chromatogr. B 830, 231-237, 2006.
  17. Cai P., Boor PJ, Khan MF, Kaphalia, BS, Ansari GAS and Konig R. Immuno- and hepato-toxicity of dichloroacetic acid in MRL+/+ and B6C3F1 mice. J Immunotoxicology 4, 107-115, 2007.
  18. Wu H, Bhopale K, Ansari GAS, and Kaphalia BS.  Ethanol-induced cytotoxicity in rat pancreatic acinar AR42J cells: Role of fatty acid ethyl esters. Alcohol Alcoholism 43, 1-8, 2008.
  19. Ping Cai, Boor PJ, Khan MF, Kaphalia, BS, Konig R and Ansari GAS. Chronic exposure to trichloroethene causes early onset of SLE-like disease in female MRL +/+ mice.  Toxicol Appl Pharmacol. 228:68-75, 2008.
  20. Kaphalia, BS. Pancreatic Toxicology. In Encyclopedia of Environmental Health. (In Press).

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