Ghulam Ansari

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Ghulam Ansari
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Academic Appointments
Appointment Organization
Professor Pathology and Human Biological Chemistry & Genetic
Professional Education
Degree Institution Field of Study Graduation Year
Doctor of Philosophy Aligarh Muslim University Specializing in synthetic organic chemistry and mass spectrometry. Thesis title "Chemical Studies in Alicyclic Systems" 1973
Master of Philosophy Aligarh Muslim University Specializing in synthetic organic chemistry and mass spectrometry. 1970
Master of Science Aligarh Muslim University Chemistry, Specialization: Organic Chemistry 1969
Bachelor of Science Aligarh Muslim University Chemistry, Physics, Mathematics 1966
Honors
Title Organization Year(s)
Co-Chaired a session entitled, “Cellular Toxicity” at 3rd World Congress on Cellular and Molecular Biology, Jena, Germany 2000
Chaired a session entitled, “Halogenated Hydrocarbons” at the 37th Annual Meeting of Society of Toxicology, Seattle, Washington 1998
Gave an invited lecture at Arabian Gulf University, Barhrain 1998
Served on NIH Study Section (ALTX – 1) 1997 - 2001
Served on board of internal advisor on program project grant submitted by Stehlin Foundation for Cancer Research, Houston, TX 1997
Invited speaker in symposium entitled, "Biomembrane and Toxicant," 2nd Congress of Toxicology in Developing Countries, New Delhi, India 1991
Consultant for United Nations Development Program entitled, "Transfer of Knowledge Through Expatriate Nationals" India 1991
Chaired a session entitled "Toxicokinetics" at the Annual Meeting of Society of Toxicology Miami Beach FL 1990
Chaired a session entitled "Xenobiotic Metabolism and Kinetics" at the V International Congress of Toxicology Brighton, England 1989
Invited speaker Department of Internal Medicine Baylor College of Medicine Houston, TX 1988
Invited speaker and chaired a session at International Conference on Biomembranes in Health and Disease India 1988
Member of peer review panel of Agency for Toxic Substances and Disease Registry for Toxicology Profiles 1988
One of the organizers of the 8th World Chromatography Conference Austria 1985
Invited to present a paper and chaired a liquid chromatography session at the 7th World Chromatography Conference Amsterdam, Holland 1984
Invited to present my work on the liver toxicity of carbon tetrachloride and participate in the 8th Saudi Medical Conference Riyadh, Saudi Arabia. 1983
Robert A. Welch Postdoctoral Fellowship University of Texas Medical Branch Galveston, TX 1976 - 1977
Postdoctoral Fellowship University of Idaho Moscow, ID 1975 - 1976
Senior Research Fellowship Council of Scientific and Industrial Research New Delhi, India 1972 - 1973
Junior Research Fellowship University Grants Commission New Delhi, India 1969 - 1972
Professional Affiliations
Society Year(s)
International Society for the Study of Xenobiotics 1989
Society of Toxicology 1985
American Society of Biochemistry and Molecular Biology 1985
American Chemical Society 1975
Research Interests

Molecular Toxicology

My research focuses on the elucidation of molecular mechanisms of toxicity of halogenated hydrocarbons, amines, unsaturated hydrocarbons and aldehydes, and development of biological markers of chemical exposure.
I have shown that xenobiotics can covalently modify fatty acids. Those xenobiotics, which are metabolized through free radicals, bind to the unsaturated part of fatty acids while xenobiotics, which contain hydroxyl on amino groups, conjugate at the carboxylic end of the fatty acids. These fatty acid conjugates cause selective organ toxicity, which may be different from the parent compounds. The mechanisms of their formation as well as of toxicity, is virtually unknown. My lab has shown that the formation of fatty acid conjugates is an enzymatic process and active organs for the synthesis are liver and pancreas. The enzymes are mainly localized in the endoplamic reticulum. I have purified and characterized enzymes responsible of the synthesis fatty acid conjugates from the liver and pancreas. Biological significance of esterifecation of fatty acid by xeno- and endobiotic is currently being pursued.
The second major emphasis in my laboratory is to develop biological markers of chemical exposure, which could provide more reliable exposure data than obtained by environmental monitoring. Over the years, I have shown that proteins and enzymes such as hemoglobin, albumin and 1-antiproteinase inhibitors, glutathione S-transferase as well as small excretory molecules such as mercapturic acids, can be used as a biological marker of chemical exposure. After thorough characterization of chemically modified proteins, efforts are directed to develop enzyme-linked immunosorbent assays to measure chemical exposure.
The third project is initiation and/or acceleration of autoimmune diseases by environmental chemicals. In this project we study the potential and mechanisms by which chemical(s) can induce/accelerate autoimmunity in autoimmune-prone mice. Model compound currently being evaluated is trichloroethene, an ubiquitous environmental contaminant.

Selected Publications
  1. Mericle, K.A., Kaphalia, B.S., Ansari, G.A.S. Modulation of fatty acid methyl esters in rats pre-treated with tri-o-tolyl phosphate. J. Toxicol. Environ. Health, Part A 67:583-593, 2004
  2. Kaphalia, B.S., Mericle, K.A., Ansari, G.A.S. Mechanism of differential inhibition of hepatic and pancreatic fatty acid ethyl ester synthase by inhibitors of serine-esterases: In Vitro and cell cultures studies. Toxicol. Appl. Pharmacol. 200:7-15, 2004
  3. Khan, M.F., Wu, X., Kaphalia, B.S., Boor, P.J., Ansari, G.A.S. Nitrotyrosine formation in splenic toxicity of aniline. Toxicology 194:95-102, 2003
  4. Khan, M.F., Wu, X., Ansari, G.A.S., and Boor, P.J. Malondialdehyde-protein adducts I the spleens of aniline-treated rats: Immunohistochemical detection and localization. J. Toxicol. Environ. Health 66:93-102, 2003
  5. Kaphalia, B.S., Ansari, G.A.S. Purification and characterization of rat pancreatic fatty acid ethyl ester synthase and its structural and functional relationship to pancreatic cholesterol esterase. Biochem. Mol. Toxicol. 17:338-445, 2003
  6. Khan, M.F., Wu, Xiaohang, Tipnis, U.R., Ansari, G.A.S. and Boor, P.J. Protein adducts of malondialdehyde and 4-hydroxnoneal in livers of iron loaded rats: quantitation and localization. Toxicology 173: 193-201, 2002
  7. Mericle, K.A., Kaphalia, B.S. and Ansari, G.A.S. Differential inhibition of hepatic, pancreatic and plasma fatty acid ethylester synthase (FAEES) by tri-o-tolylphosphate in rats. Toxicol. Appl. Pharmacol. 179: 119-125, 2002
  8. Khan, M.F., WU, X., Ansari, G.A.S. Anti-malondealdehyde antibodies in MRL+/+mice treated with trichloroethene and dichloroacetyl chloride: Possible role of lipid peroxidation in autoimmunity. Toxicol. Appl. Pharmacol. 170: 88-92, 2001
  9. Kaphalia, B.S., Ansari, G.A.S. Purification and characterization of rat hepatic microsomal low molecular weight fatty acid ethyl ester synthase and their relationship to carboxylesterases. Biochem. Mol. Toxicol. 15: 165-171, 2001
  10. Green, S.M., Khan, M.F., Kaphalia, B.S., Ansari, G.A.S. Immnohistochemical localization of protein adducts of tetrachloreoethene in mice. J. Toxicol. Environ. Health 63: 145-157, 2001
  11. Khan MF, Wu X and Ansari GAS. Nitrosobenzene and splenic toxicity of aniline. J. Toxicol,Environ, Health 60, 263-273, 2000
  12. Awasthi S, Pandya W, Singhalm SS, Lin JJ, Thiviyanathan V, Seifert WE, Awasthi YC and Ansari GAS. Curcumin-glutathione interactions and the role of human glutathione S-transferase P1-1. Chem.-Biol,Inter. 128:19-38,2000.
  13. Khan MF, Wu X, Boor PJ and Ansari GAS. Oxidative modification of lipids and proteins in aniline-induced splenic toxicity. Toxicol. Sci. 48:130-140, 1999
  14. Kaphalia BS, Khan MF, and Ansari GAS. Fatty acid anilides: In vivo formation and relevance to toxic oil syndrome. J. Biochem. Mol. Toxicol. 13:269-277, 1999
  15. Kaphalia BS, Green SM, Ansari GAS. Fatty acid ethyl and methyl ester syntheses and fatty acid anilide synthase in HepG2 and AR42J cells: Interrelationships and inhibition by tri-o-tolyl phosphate. Toxicol. Appl. Pharmacol. 159:134-141, 1999
  16. Kaphalia BS and Ansari GAS. Single step thin-layer chromatographic method for quantitation of enzymatic formation of fatty acid anilides. J. Chromatogr. B (Biomedical Sciences and Application) 705:269-275, 1998
  17. Kadjipavlou AG, Simmons JW, Yang JP, Ansari GAS, Kaphalia BS, Simmons DJ, Nicodemus CL, Lane R and Esch O. Torsinal injury resulting in disc degeneration: An in vivo rabbit model. J. Spinal Disorder 11:312-317, 1998
  18. Khan MF, Green SM, Ansari GAS and Boor PJ. Phenylhydroxylamine: Role in aniline-associated splenic oxidative stress and induction of subendocardial necrosis. Fundam. Appl. Toxicol. 42:64-71, 1998
  19. Kaphalia BS, Fritz RR and Ansari GAS. Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (Pl 6.1). Chem. Res. Toxicol. 10:211-218, 1997
  20. Khan MF, Boor PJ, Gu Y, Alcock NW and Ansari GAS. Oxidative stress in the spleenotoxicity of aniline. Fundam. Appl. Toxicol. 35:22-30, 1997.
  21. Khan MF, Kaphalia BS and Ansari GAS. Time-dependent autoimmune response of dichloroacetyl chloride in female MRL +/+ mice. Immunopharmacol. Immunotoxicol. 19:296-277, 1997
  22. Awasthi S, Bajpai KK, Singhal SS, Piper JT, Ballatore A, Seifert WE, Ansari GAS, Awasthi YC. Interactions of melphalan with glutathione and the role of glutathione S transferases. Drug Met. Dispos. 24:371-373, 1996
  23. Kaphalia BS, Khan MF, Carroll RM, Aronson J and Ansari GAS. Subchronic toxicity of 2-chloro-and 2-bromoethanol in rats. Res. Commun. Pharmacol. Toxicol. 1:173-186, 1996
  24. Kaphalia BS, Ghanayem BI and Ansari GAS. Non-oxidative metabolism of 2-butoxyethanol via fatty acid conjugation in Fisher 334 rats. J. Toxicol. Environ. Health 49:101-117, 1996
  25. Khan MF, Kaphalia BS, Ansari GAS. Erythrocyte-aniline interaction leads to their accumulation and iron deposition in rat spleen. J Toxicol Environ Health 44:415-521, 1995
  26. Khan MF, Boor PJ, Kaphalia BS, Alcock NW, Ansari GAS. Hematopoietic toxicity of linoleic acid anilide: Importance of aniline. Fundam Appl Toxicol 25:224-232, 1995
  27. Ansari GAS, Kaphalia BS, Khan MF. Fatty acid conjugates of xenobiotics. (invited review) Toxicol Lett 75:1-17, 1995
  28. Khan MF, Srivastava SK, Singhal SS, Chaubey M, Awasthi S, Petersen DR, Ansari GAS, Awasthi YC. Iron induced lipid peroxidation in rat liver is accompanied with preferential induction of glutathione S-transferase 8-8 isozyme. Toxicol Appl Pharmacol. 131:63-72, 1995
  29. Zeng FY, Kaphalia BS, Ansari GAS, Wiegel PH. Fatty acylation of the rat asialoglycoprotein receptor: the three subunits form active receptors contain covalently bound palmitate and stearate. J Biol Chem 270:21382-21387, 1995
  30. Khan MF, Kaphalia BS, Prabhakar BS, Kanz MF, Ansari GAS. Trichloroethene - induced autoimmune response in female MRL +/+ mice. Toxicol Appl Pharmacol 134:155-160, 1995
  31. Kaphalia BS, Carr JB, Ansari GAS. Increased endobiotic fatty acid methyl esters following exposure to methanol. Fundam Appl Toxicol. 28:264-273, 1995
  32. Thakore KN, Gan JC, Ansari GAS. Rapid plasma clearance of albumin-acrolein adduct in rats. Toxicol Lett 71:27-37, 1994
  33. Khan MF, Kaphalia BS, Boor PJ, Ansari GAS. Subchronic toxicity of aniline hydrochloride in rats. Arch Environ Contam Toxicol 24:368-374, 1993
  34. Ahmad F, Kaphalia BS, Khan MF, Ansari GAS. Fatty acid anilides: a new conjugation pathway of aniline. Biochem Arch 9:111-118, 1993
  35. Awasthi S, Srivastava SK, Ahmad F, Ahmad H, Ansari, GAS. Interactions of glutathione S-transferase with ethacrynic acid and its glutathione conjugate. Biochem Biophys 1164: 173-178, 1993
  36. Boor PJ, Trent MB, Lyles GA, Tao M, Ansari GAS. Methylamine metabolism to formaldehyde by vascular semicarbazide-sensitive amine oxidase. Toxicology 73:251-258, 1992
  37. Kaphalia BS, Ansari GAS. Covalent binding of ethylene dibromide and its metabolites to albumin. Toxicol Lett 62:221-230, 1992
  38. Kaphalia BS, Khan MF, Boor PJ, Ansari GAS. Toxic response to repeated oral administration of 2-chloroethyl linoleate in rats. Res Commun Chem Path Pharmacol 76:209-222, 1992
  39. Thakore K, Gan JC, Oandasan A, Ansari GAS. Quantitation of blood protein adducts of acrolein by tritiated borohydride reduction. Toxicol Methods 2:295-305, 1992
  40. Kanz MF Kaphalia L, Kaphalia BS, Ramagnoli E, Ansari GAS. Methylene dianiline: Acute toxicity and effect on biliary function. Toxicol. Appl. Pharmacol. 117:89 92, 1992
  41. Khan MF, Kaphalia BS, Ansari GAS. Toxic response to linoleic acid anilide in female rats. Res Commun Chem Pathol Pharmacol 77:241-244, 1992
  42. Sharma R, Gupta S, Singhal SS, Ansari GAS, Awasthi YC. Glutathione S-transferase catalyzed conjugation of 9,10-epoxysteric acid with glutathione. J Biochem Toxicol 6: 147-153; 1991
  43. Boor PJ, Khan MF, Kaphalia BS, Jerrells TR, Ansari GAS. Synergistic vascular toxicity and fatty acid anilides in the toxic oil syndrome. J Am Coll Cardio 18: 1824-1828; 1991
  44. Khan MF, Kaphalia BS, Palafox A, Jerrells TR, Ansari GAS. Toxicity of oleic acid anilide in rats. Arch Environ Contam Toxicol 21: 571-577; 1991
  45. Khan MF, Kaphalia BS, Palafox A, Jerrells TR, Ansari GAS. Heated linoleic acid anilide: Toxicity and relevance to toxic oil syndrome. Toxicol 68: 143-155; 1991
  46. Ahmed AE, Ansari GAS, Dencker L, Ullberg S. Differential distribution and placental transport of 2- and 3-t[Methyl-14C] butyl-4-hydroxyanisole (BHA) in pregnant mice. Fund Appl Toxicol 16: 356-364; 1991
  47. Peterson JW, Reitmeyer JC, Jackson CA, Ansari GAS. Protein synthesis is required for cholera toxin-induced stimulation of arachidonic acid metabolism. Biochem Biophys Acta 1092: 79-84; 1991
  48. Dayal HH, Brodwick M, Morris R, Baranowski T, Trieff N, Harrison JA, Lisse JR, Ansari GAS. A community-based epidemiologic study of health sequelae of exposure to hydrofluoric acid (HF). Ann Epidemiol 2: 213-230; 1991
  49. Kaphalia BS, Khan MF, Ansari GAS. Reduced activities of serum lactate dehydrogenase and aminotransferases due to an oral administration of 2-chloroethyl linoleate in rats. Bull Environ Contam Toxicol 48: 308-312; 1991
  50. Kaphalia BS, Bhat HK, Khan MF, Ansari GAS. Tissue distribution of monochloroacetic acid and its binding to albumin in rats. Toxicol Ind Hlth 8: 53 61; 1991
  51. Gan JC, Oandasan A, Ansari GAS. In vitro covalent modification of serum albumin by acrolein. Chemosphere 23: 939-947; 1991
  52. Bhat HK, Asimakis GA, Ansari GAS. Uncoupling of oxidative phosphorylation in rat liver mitochondria by chloroethanols. Toxicol Lett 59: 203-211; 1991
  53. Singhal SS, Gupta S, Saxena M, Sharma R, Ahmad H, Ansari GAS, Awasthi YC. Purification and characterization of glutathione S-transferases from rat pancreas. Biochem Biophys Acta 1079: 285-292; 1991
  54. Khan MF, Kaphalia BS, Ansari GAS. Heated linoleic acid anilide reduces serum enzyme activities in rats. Res Commun Chem Pharmacol 17: 240-253; 1991
  55. Kaphalia BS, Ansari GAS. Rapid chromatographic analysis of fatty acid anilides suspected of causing toxic syndrome. Anal Toxicol 14: 1-5; 1991
  56. Bhat HK, Kanz MF, Campbell GA, Ansari GAS. Ninety day subchronic toxicity study of chloroacetic acids in rats. Fund Appl Toxicol 17: 240-253; 1991
  57. Kaphalia BS, Ansari GAS. Trichloromethyl Peroxyl Radical (Cl300.) Mediated Oxidation of Cholesterol. Chemosphere 20: 5-11; 1990.
  58. Ahmad H, Medh RH, Singh SV, Caccuri AM, Ansari GAS, Awasthi YC. Anionic glutathione S-transferases of human erythrocytes, placenta and lung: Evidence of structural differences. Enzyme 42: 129-135; 1990
  59. Bhat HK, Ahmed AE, Ansari GAS. Toxicokinetics of monochloroacetic acid: A whole-body autoradiography study. Toxicology 63: 35-43; 1990
  60. Bhat HK, Ansari GAS. Cholesterol ester hydrolase mediated conjugation of haloethanols with fatty acids. Chem Res Toxicol 3: 311-317; 1990
  61. Sharma R, Gupta G, Ahmad H, Ansari GAS, Awasthi YC. Stimulation of human erythrocyte membrane ATPase by glutathione conjugates. Toxicol Appl Pharmacol 104:421-428; 1990
  62. Bandi ZL, Ansari GAS. Isolation of hydroxy fatty acids from livers of carbon tetrachloride treated rats by thin layer chromatography. J Chromatogr 475: 461-466; 1989
  63. Britt SG, Ansari GAS. Formation of lipid abducts by carbon tetrachloride. Clin Chem Enzymol Commun 1: 305-311; 1989
  64. Bhat HK, Ansari GAS. Chromatographic separation of cholesteryl acetate and its chloro analogues. J Chromatogr 462: 467-470; 1989
  65. Sanduja R, Ansari GAS, Boor PJ. 3-Hydroxypropylmercapturic acid: A biologic marker of exposure to allylic and related compounds. J Appl Toxicol 9: 235-238; 1989
  66. Bhat HK, Ansari GAS. Covalent Interaction of Chloroacetic and Acetic Acids with Cholesterol. J Biochem Toxicol 4: 189-193; 1989
  67. Gan JC, Ansari GAS. Inactivation of plasma 1-proteinase inhibitor by acrolein: Adduct formation with the lysine and histidine residues. J Biochem Toxicol 2: 137-145; 1989
  68. Kaphalia BS, Ansari GAS. Covalent modification of hepatic microsomal lipids of rats by carbon tetrachloride. Mol Toxicol 2: 199-213; 1989
  69. Kaphalia BS, Ansari GAS. Hepatic Fatty Acid Conjugation of 2-Chloroethanol and 2 romoethanol in Rats. J Biochem Toxicol 4: 183-188; 1989
  70. Bhat HK, Ansari GAS. Improved Separation of Lipid Esters by Thin-Layer Chromatography. J Chromatogr 483: 369-378; 1989
  71. Ansari GAS, Gan JC, Barton BK. In vitro inactivation of plasma 1 proteinase inhibitor by exposides and 1,2-dihaloethanes. Arch Environ Contam Toxicol 17: 537-542; 1988
  72. Bandi ZL, Ansari GAS. Separation of rat liver phospholipids from polar nonphospholipids by thin layer chromatography. J Chromatogr 435: 513-517; 1988
  73. Ahmad H, Singh SV, Medh RD, Ansari GAS, Kurosky K, Awasthi YC. Glutathione S-transferases: Tissue specific expression in lens, cornea, and retina. Arch Biochem Biophys 266: 416-426; 1988
  74. Ansari GAS, Gan JC, Barton BK. Synergistic inactivation of plasma 1 proteinase inhibitor by aldehydes of cigarette smoke with styrene oxide and 1,2-dichloroethane. Arch Environ Contamin Toxicol 17: 533-536; 1988
  75. Singh SV, Leal T, Ansari GAS, Awasthi YC. Purification and characterization of glutathione S-transferases of human kidney. Biochem J 246: 179-186; 1987
  76. Gan JC, Ansari GAS. Plausible mechanism of inactivation of plasma 1-proteinase inhibitor by acrolein. Res Commun Chem Pathol Pharmacol 55: 419-422; 1987
  77. Upreti RK, Farooqui MYH, Ahmed AE, Ansari GAS. Toxicokinetics and molecular interaction of [14C]-formaldehyde in rats. Arch Environ Contam Toxicol 16: 263 273; 1987
  78. Ansari GAS, Kaphalia BS, Boor PJ. Selective pancreatic toxicity of palmitoylpentachlorophenol. Toxicology 46: 57-63; 1987
  79. Sanduja R, Ansari GAS, Boor PJ. Simultaneous determination by myocardial creatinine phosphate and adenine nucleotides by reversed-phase high performance liquid chromatography. Biomed Chromatogr 2: 156-158; 1987
  80. Kaphalia BS, Ansari GAS. 2-Chloroethylstearate: A novel in vivo conjugate of 2 chloroethanol. Bull Environ Contam Toxicol 39: 835-842; 1987
  81. Boor PJ, Sanduja R, Nelson TJ, Ansari GAS. In vivo metabolism of the cardiovascular toxin, allylamine, to acrolein. Biochem Pharmacol 36: 4347-4353; 1987
  82. Ansari GAS, Singh SV, Gan JC, Awasthi YC. Human erythrocyte glutathione S-transferase: A possible marker of chemical exposure. Toxicol Lett 37: 57-62; 1986
  83. Gan JC, Ansari GAS. Non-oxidative inactivation of plasma-proteinase inhibitor by carbonyl compounds found in cigarette smoke. Res Commun Sub Abuse 7: 59-69; 1986
  84. Boor PJ, Ansari GAS. A high performance liquid chromatographic method for quantitation of acrolein in biological samples. J Chromatogr 375: 159-164; 1986
  85. Ansari GAS, James GP, Hu LA, Reynolds ES. Polychlorinated aromatic hydrocarbon residues in adipose tissue of residents of the Texas Gulf Coast. Bull Environ Contam Toxicol 36: 311-316; 1986
  86. Bandi ZL, Ansari GAS. High performance liquid chromatographic analysis of saturated fatty acid mixtures containing positional isomers of various chain lengths. J Chromatogr 363: 402-406; 1986
  87. Farooqui MYH, Upreti RK, Ahmed AE, Ansari GAS. Influence of intraperitoneally administered formaldehyde on bile production and glutathione levels in rats. Res Commun Chem Pathol Pharmacol 53: 233-236; 1986
  88. Singh SV, Ansari GAS, Awasthi YC. Anion exchange high performance liquid chromatography of glutathione S-transferases: Separation of the minor isozymes of human erythrocytes, heart, and lung. J Chromatogr 361: 337-345; 1986
  89. Ansari GAS, Hendrix PY. Tissue distribution and pharmacokinetics of 3 tert-(methyl-14C)butyl-4-hydroxyanisole in rats. Drug Metab Disp 13(5): 535-541; 1985
  90. Ansari GAS, Britt SG, Reynolds ES. Isolation and characterization of palmitoylpentachlorophenol from human fat. Bull Environ Contam Toxicol 34: 661 667; 1985
  91. Cummings SW, Ansari GAS, Guengerich FP and Prough RA. The metabolism of 3-t-butyl-4-hydroxyanisole by microsomal fractions and isolated rat hepatocytes. Cancer Res 45:5617 5624; 1985
  92. Ansari GAS, Hendrix PY. Rapid and convenient separation of pentachlorophenol from human fat using silica Sep-pak cartridges. J Chromatogr 346: 435-439; 1985
  93. Ansari GAS. Separation of butylated hydroxyanisole isomers by glass capillary gas chromatography. J Chromatogr 291: 421-423; 1984
  94. Ansari GAS. High performance liquid chromatographic separation of the isomers of butylated hydroxyanisole. J. Chromatogr. 262: 393-396; 1983
  95. Ansari GAS, Patel NT, Fritz RR, Abell CW. Purification of human platelet monoamine oxidase B by high performance liquid chromatography. J Liq Chromatogr 6: 1407-1419; 1983
  96. Ansari GAS, Walker, RD, Smart VB, Smith LL. Further investigations of mutagenic cholesterol preparations. Food Chem. Toxicol. 20: 35-41; 1982
  97. Ansari GAS, Moslen MT, Reynolds ES. Evidence for in vivo covalent binding of •CCl3 derived from CCl4 to cholesterol of rat liver. Biochem Pharmacol 31: 3509-3510; 1982
  98. St Claire RL, Ansari GAS, Abell CW. Determination of hydroisoquinolines by reverse-phase liquid chromatography with gradient elution and amperometric detection. Anal Chem 54: 186-189; 1982
  99. Forkert PG, Custer EM, Alpert AJ, Ansari GAS, Reynolds ES. Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene: Index of airway injury. Expt Lung Res 4: 67-77; 1982
  100. Hu LA, Ansari GAS, Moslen MT, Reynolds ES. Efficient clean-up of fat samples by Sep-pak cartridges for polybrominated biphenyl (PBB) analysis. J Chromatogr 241: 419 422; 1982
  101. Ansari GAS, Smith LL. High performance liquid chromatography of cholesterol autoxidation products. J Chromatogr 175: 307-315; 1979
  102. Smith LL, Smart VB, Ansari GAS. Mutagenic cholesterol preparations. Mutat. Res. 68:23-30; 1979
  103. Ansari GAS, Smith LL. The oxidation of cholesterol by hydroxyl radical. Photochem. Photobiol. 30: 147-150; 1979
  104. Smith LL, Kulig MJ, Miller D, Ansari GAS. Oxidation of cholesterol by dioxygen species. J Am Chem Soc 100: 6206-6211; 1978
  105. Ansari GAS, Smith LL. Steroid Metabolism XLI. Cholesterol A-ring autoxidation. Chem. Phys Lipids 22: 55-62; 1978
  106. Ahmad MS, Mushfig M, Asif MF, Ansari GAS. Mass spectral studies on steroidal compounds-IV, 6-oxa-B-homo-5-cholestan-7-one and its derivatives. J Practisch Chemie 317: 1049-1053; 1978
  107. Khan MF, Wu X, Kahalia BS, Boor PJ and Ansari GAS. Acute hematopoietic toxicity of aniline in rats. Toxicol. Lett. 92:31-37, 1977
  108. Ahmad MS, Mushfig M, Ansari GAS, Waris F. Mass spectral studies on steroidal compounds-V, rings B and A seco 5-keto compounds in the cholestane series. Org Mass Spectrom 8: 1-10; 1974
  109. Ahmad MS, Ansari GAS. Mass spectral studies on steroidal compounds-VI, 3-hydroxyethers and related compounds in the cholestane series. Canad J Spectros 19:105-109; 1974
  110. Ahmad MS, Ansari GAS. Mass spectral studies on steroidal compounds-VIII, 6-hydroxyethers and related compounds in the cholestane series. Canad J Spectros 19:133 136; 1974
  111. Ahmad MS, Ansari GAS, Waris F. 3-Acetoxy-6-oxa-B-homocholest-4-en-7-one and related compounds. Ind J Chem 12: 455-457; 1974
  112. Ahmad MS, Ansari GAS. Mass spectral studies on steroidal compounds-I, steroidal 6 one ethylene acetals. Org Mass Spectrom 6: 1095-1108; 1973
  113. Ahmad MS, Ansari GAS. Hydrogenolysis of 6-bromo-3,3-ethylenedioxycholest-5-ene and 6,6(1'-methylethyl ethylenedioxy)-3-,5-cyclo-5-cholestane. Ind J Chem 10:1052 1056; 1972
  114. Ahmad MS, Logani SC, Ansari GAS. Lithium aluminum hydride-aluminum chloride reduction of 5-cholestan-6-one and 3-chloro-5-cholestan-6-one ethylene acetals. Ind J Chem 9: 1329-1331; 1971
  115. Ahmad MS, Ansari GAS, Pillai NK. Anomalous behavior of -(5,6)-lactone of 3-acetoxy-B-nor-5-cholestan-5-ol-6-carboxylic acid toward methanolic hydrogen chloride. Ind J Chem 8: 758-759; 1970

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