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Name:
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Date: |
Emp
ID:
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Clinic / Unit:
TSM
Name: |
Preceptor(s): |
CERT EXPIRES:
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POC
Test: Quidel QuickVue Influenza A+B |
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AFTER
TAKING ONLINE TEST AND READING THE POLICY,
EMPLOYEE PERFORMS A TEST AND MARKS RESULTS BELOW.
THE PRECEPTOR OR TSM GRADES RESULTS FOR ACCURACY. |
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1. Reads entire
Policy/Procedure
2. Completes and passes
written test (Passing criteria is 100%)
3. Return demonstration |
TEST SCORE:
0
Test taken on:
5/26/2013 |
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CRITICAL
POINTS
FROM POLICY AND PROCEDURE
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OPERATOR
INITIALS |
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a.
Specimen:
Acceptable specimens for the QuickVue Influenza A+B Test
are: a nasal swab, nasal wash, or nasal aspirate.
Specimens should be tested as soon as possible after
collection.
Specimens may be stored refrigerated (2-8°C), or at room
temperature (15-30°C) in a clean dry closed container
for up to eight (8) hours prior to testing. DO NOT use
any kind of transport media to store or transport
samples.
Unacceptable specimens:
Specimens taken from other sites i.e. saliva, sputum,
urine. Samples collected with swabs not provided by Quidel test kit |
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b. Patient Testing:
1. Dispense one reagent tube of Extraction Reagent
Solution into an Extraction Tube and gently swirl to
dissolve contents.
2. Place patient nasal swab into extraction tube. Swabs
MUST stay in the extraction solution for 1 minute prior
to removal.
3. Roll swab at least three times while pressing the
head against the bottom and side of the extraction tube.
Roll the swab head against the inside of the Extraction
Tube as you remove it.
4. Dispose of the swab in accordance with your biohazard
waste disposal protocol.
5. Place the test strip into the extraction tube with
the arrows on the test strip pointing down. Do not
handle or move the test strip until the test is complete
and ready for reading.6. After ten minutes has elapsed, hold the test strip
with the arrows pointed down to interpret test results.
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c. Interpretation of Results:Postiive Result:
At 10 minutes, ANY shade of pink-to-red Test line forms
AND appearance of a blue procedural Control Line
indiciates a positive result for the presence of viral
antigen
Negative Result:
At 10 minutes, the appearance of ONLY the blue
procedural Control Line indicated the sample is negative
for viral antigen.
A negative result should be reported as a presumptive
negative for the presence of influenza antigen and a
second nasal swab should be submitted to the MICRO lab
for viral culture
Invalid Result:
If at 10 minutes, the blue procedural Control Line does
not appear, even if any shade of pink-to-red Test line
appears, the result is considered invalid. If at 10
minutes the background color does not clear and it
interferes with the reading of the test, the result is
considered invalid. If the test is invalid, a new test
should be performed with a new patient sample and a new
test strip. |
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c.
Quality Control:
Document appearance of onboard Control Line on first
sample on day of patient testing. Also 2 Liquid Controls
must be documented when a new Lot Number of kits is
opened. |
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d. Reagent Storage:
Store test kit at room temperature (15-30°C) and kept
away from direct sunlight. Do NOT freeze and Do NOT use
kits beyond the expiration date printed on the outside
of the box. |
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Sample: _________________
Result: ________________
Pass: _____Yes _____No |
VALIDATOR:___________ |
