Real-Time PCR Core Facility

The real-time PCR Core Facility, directed by Lisa Elferink, Ph.D., offers a full complement of real-time PCR services for UTMB departments and centers. These services include primer and probe design and order, preparation of real-time PCR reactions and data analyses for gene expression (DNA and RNA samples), MicroRNA assays as well as allelic discrimination. The PCR core facility has the following major equipment available: Applied Biosystems (ABI) PRISM 7000 sequence detector with a Dell Pentium III laptop computer; a Dell Pentium IV desktop computer installed with the ABI PRISM 7000 SDS (sequence detection system), PrimerExpress and file builder software; a Hewlett Packard DeskJet 970 cxi printer; a Beckman GS-6R centrifuge with a GH3.7 rotor; a VSMC-13 mini centrifuge; a refrigerator and a ‑20 C freezer.