logo
red bar

Home
Mission
Investigators
Transgenic Mouse Facility
Real-time PCR Core Facility
Human Tumor Bank Core Facility
 Career Opportunities
 Funding Opportunities
 Graduate Training in Cancer
   Cancer Cell Biology Track
   SCCCB Predoctoral Fellowship
   Cancer Center Training Grant
Seminars
In the News
 Related Links
 Site Index
 Software - Molecular Biology Tools
 UTMB Cancer Center
 UTMB Home Page

Real-Time PCR Core Facility

Services and Procedures

General InformationWhy use the Real Time PCR core?Services and Procedures
Service Request Forms
Assay Transfer AgreementPublished DataSatisfaction Survey


Dr. Huiping Guo

Thank you for interest in our real time PCR core. Our procedure is very simple:

  1. Email gene name(s) and genebank accession number(s) to nhe@utmb.edu. Indicate whether to measure RNA(cDNA) or genomic DNA samples and specific region if your target is not general gene. You are also welcomed to discuss your project (such as transgene, knockout, SiRNA, SNP, microRNA, etc.) in person.
  2. We will notify you the cost of the assay(s)
  3. You are required to confirm the ordering by sending ISC# as well as your department and supervisor’s name.
  4. We will design and place the order and notify you by email when it is received (approximately 3~4 business days for inventory assays and 2~3 weeks for non-inventory and custom assays).
  5. Bring your well-cleaned (include totally removed DNA contamination from your RNA samples) samples to the Real Time PCR Core Facility, MRB 9.150.
    Bring cDNA samples, do RT with random hexamers; you can bring RNA samples if your target gene expression is high.
  6. Fill out the sample sheet.

The results will be emailed to you within 5 business days.

Please send a copy of your publication as a record.

red bar
This site is published by John Helms for the Sealy Center for Cancer Cell Biology.
Copyright © 2003
The University of Texas Medical Branch. Please review our Privacy Policy and Internet Guidelines.
Last reviewed: November 5, 2008.