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What are  Stem Cells?
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STEM C= ELLS ARE FOUND IN MULTICELLULAR ORGANISMS.
nHave ability to grow into any one of the body̵= 7;s more than 200 cell types. nRetain the ability to divide throughout life and g= ive rise to highly specialized cells.
<= span style=3D'font-size:81%'>nTake the place of = cells that die or are lost.
<= span style=3D'font-size:81%'>nCan divide in cult= ure over indefinite periods of time.
<= span style=3D'font-size:81%'>nDifferentiate when subjected to biochemical signals.
nPluripotent cells = are the most versatile cells and are present in the first days after an egg is fertilized.
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DjICoYMSJmoCNSPZygEBADs= ------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0003.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
STEM CELLS
<= span style=3D'mso-special-format:bullet;color:#CCFF33;position:absolute;left:-3= .85%; top:.39em;font-family:Wingdings;font-size:70%'>nStem cells are made= in the bone marrow.
<= span style=3D'mso-special-format:bullet;color:#CCFF33;position:absolute;left:-3= .96%; top:.39em;font-family:Wingdings;font-size:70%'>nStem cells can be encouraged to form a specific type of cell. <= /div>
nIt is possible for = stem cells to transform into heart, nerve, or other types of cells.
<= span style=3D'mso-special-format:bullet;color:#CCFF33;position:absolute;left:-4= .44%; top:.39em;font-family:Wingdings;font-size:70%'>nYoung stem cells can transform throughout life into functional components of blood (i.e., red and white cells). 
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0004.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
HUMAN EMBRYONIC STEM CELLS
nHuman embryonic stem cells derived from = embryo prior to differentiation.
nBuilding material – can be continually = reproduced.
nMay be used a super repair kit for the body. <= /span>
nLines are derived from individual cells.
nCan be converted into neural cells but need to = be pluripotential to produce all types of <= /span>neurological cellular material.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0005.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
PLURIPOTENT STEM CELLS
nAlthough human pluripotent stem cells are <= /span>= derived from embryos or fetal tissue such stem = cells are not themselves embryos.
nAll tissues develop from the three primary germ layers – ectoderm, mesoderm, and endoderm.&#= 13;
nThe truly self replicating cell divides to form ce= lls = indistinguishable from itself. <= /div>
nStem cells are unspecialized cells capable of = self renewal and can be induced to become <= /span>= organ specific cells.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0006.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
HUMAN EMBRYONIC STEM CELLS
nDerived from the embryo which can be grown in the laboratory. = nCan b= e taken from in-vitro fertilization embryos. nSomat= ic or “adult” stem cells can be reprogrammed genetically = to assume a stem cell like state.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0007.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
STEM CELL LINES
n100 – 150 cells in the early embro are = pluripotential cells. = nThe science is controversial from an ethiccal and theological standpoint.
<= span style=3D'font-size:88%'>nOnly stem cell lin= es can self renew.
<= span style=3D'font-size:88%'>nSome cells on the registry do not differentiate.
nMany of the initia= lly approved stem cell lines were ineffective.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0008.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
STEM CELL LINES
nEarly cell differentiation can produce progenitors.
nProgenitor cells are the raw material for heart or nerve tissue and can be grown on a scaffold.
nThe scaffold can then be removed or destroyed, leaving transplantable active differentiated cells.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0009.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
NATIONAL STEM CELL BANK
nNational Stem Cell Bank – closed February 2010.
nCells now obtainable from “Wisconsin <= span style=3D'position:absolute;top:49.5%;left:8.42%;width:89.88%;height:6.75%'= >International stem Cell Bank” – WiCell Research Institute. <= /span>
nAvailable
nStem Cell Lines SA0= 1, SA02, ES01, etc.
nModified Cell Lines= H9, CRE, LOX-P.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0010.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
NATIONAL STEM CELL BANK
<= span style=3D'visibility:hidden'>n
<= span style=3D'visibility:hidden'>n
nNIH S= tem Cell United analyzed and compared 21 hESC lines. <= span style=3D'position:absolute;top:62.0%;left:8.42%;width:84.26%;height:6.75%'= >nNIH s= upports short term training programs.
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TYPICAL CULTURE PROTOCOL
nPlace cells in gelatin well plate.
nInactivate with mouse embryonic fibroblasts or irradiate.
nManually remove differentiating cell clumps.
nWash cells with PBS.
nCollect colonies and re-suspend in hESC medium.
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THE PROMISE OF STEM CELLS
nBirth defects and cancer are due to = = abnormalities of cellular differentiation.
nResearch is still preliminary. <= /div>
n2009 – Geron (California) received FDA clearance to begin first human clinic trial of cel= ls derived from ESC.
nUses:  reconstituting the immune system in leukemia, lymphoma, and autoimmune <= span style=3D'position:absolute;top:77.5%;left:8.42%;width:84.26%;height:6.0%'>= diseases.
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ETHICAL ISSUES
nBush Administration restricted lines available.= 3;
nPresident’s Council on Bioethics.
nBioethics Advisory Committee (BAC) Singapore <= /span>
nEuropean Union Report on Human Embryonic = Stem Cell Research
nMarch 2009 Obama Administration Executive <= /span>= Order “Removing Barriers to Responsible = Scientific Research Involving Human Stem Cells”
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THE BLASTOCYST
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nThe 3= -5 day old embryo
nInner= cells give rise to the entire body, including all specialized tissues.
n
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STEM CELL RENEWAL
<= span style=3D'visibility:hidden'>n
nEmbry= onic stem cells can go on reproducing in the long term. nProli= feration in the laboratory can give rise to millions of cells over years.
<= span class=3DBB style=3D'position:absolute;left:-3.87%;top:.39em'>nMost non-embryonic cells cannot do this.
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DIFFERENTIATION
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-3.79%;top:.39em'>nInter= action of internal and external signals
nDNA a= cquires epigenetic markers that restrict DNA expression in the cell= and can be passed on through cell division. nLearn= ing more about signaling is the focus of much current research.
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ADULT STEM CELLS
nUndifferentiated cells found within a particular organ
nRestricted in type of cell produced
nHemopoietic stem cell only produces cells for the blood stream.
nMaintain and repair tissue.
nProbably not from inner layer of blastocyst.
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ADULT STEM CELLS
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nExist= in brain and heart.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nResid= e in stem cell niches.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nRespo= nd to cell culture.
nIdent= ified by molecular markers and transplantation of cells in animals.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nTrans= differentiation can occur.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nCells= can be reprogrammed.
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ADULT STEM CELLS
n
nMULTIPOTENT – different types of cell with the same basic cell type.
nEmbryonic Stem Cells
nPLURIPOTENT can run into all of the body’s cell types except egg and sperm cells.
nEmbryonic Stem Cells have been induced to produce insulin.
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SOMATIC NUCLEAR TRANSFER
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>n̶= 0;Therapeutic cloning”
nAllows production of embryonic without using sperm to fertilize an egg. nCell lines could be made in this way that are specific to a patient or disease process.
------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/slide0021.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" What are Stem Cells?
STEM = CELLS – CELLULAR BIOLOGY
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nStem = cells remain an enigma.
nThey = carry the same DNA as regular body cells.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nHave a uniquely flexible identity.
nCan d= evelop into a wide variety of different tissues.
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HOW DO= THEY KEEP THEIR OPTIONS OPEN?
nEpigenetics – chemical changes that affect how genes behave without altering their DNA.
nDepends on METHYLATION
nBody cells – Methylation occurs where = cytosine and quanine are together
nStem cells – 25% of methylation occurs outside these zones.
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PLURIPOTENT STEM CELLS
nBody cells that have been reprogrammed with the = ability to grow into multiple types of tissue. = ;
nThese induced cells have a pattern of methylation = similar to that of embryonic stem cells.
nThe arrangement of methyl groups may be the key feature in determining the range of a cell’s possible future forms. =
nThe epigenetic map may help to determine whether <= /span>= induced stem cells are as versatile as embryonic <= /span>ones.
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STEM CELL TRANSFER
nHow transferable are cells?
nConflicting evidence:
nDormant gene expression programs can be dominantly awakened in differentiated cells by fusion of different pairs= of cell types.
nLineage conversions can be effected simply through the introduction of defined transcription factors.
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STEM CELL TRANSFER
n
nConflicting evidence:
nIn a number of species, transfer of nuclei from = both embryonic and adult somatic cell types can lead to the formation = of all three germ layers and even generation of new animals. <= /span>
nThis demonstrates that the identity of differentiated cells can be reversed.
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CHANGING LINEAGE
nFunctionally differentiated cells can change lineage.
nFetal blood cells in the aorta have their development influenced by stress factors, minimizing flow.
nTranscription factors are necessary.
nBad T-cell progenitors can be converted into functional macrophages.
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LINEAGE:
INSTRUCTIVE TRANSCRIPTION F= ACTORS
<= span style=3D'visibility:hidden'>n
n̶= 0;Step on the accelerator” – induce a new gene expression.&= #13;n̶= 0;Put on the brakes” – inactivate key regulators.  Cell intrinsic and extrinsic factors are at work.
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CAN CE= LL TYPE BE DIRECTLY PROGRAMMED?
n
ne.g., B- cells
nTranscription factor can convert to macrophages.
nCan they be induced to become islet cells? <= /div>
nInconclusive evidence
nCell population concentrations and defined genes may be necessary.=
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GENER= ATING CELLS “A LA CARTE”
nHigh frequency transitions are necessary
nInducible, sequential, and graded expression of transcription factors
nModulation of micro RNAs
nExocine pancreas cells can be converted into islet cells in mice.
nCustom designing in humans is a long way off. <= /u>
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REPAI= R OF THE INJURED SPINAL CORD
n
nSpinal cord trauma
nCell death begins at 12 hours
nCenter of cord first (gray matter)
nWhite matter is critical
n
n
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REPAI= R OF THE INJURED SPINAL CORD
n
nCure is not possible, but partial repair can provide disproportionately large gains.
nBowel and bladder control is major requirement and occurs peripheral fi= bres in the cord.
n
n
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TREATME= NT OF SPINAL CORD DEFECTS
<= span style=3D'font-size:88%;visibility:hidden'>n= 3;
<= span style=3D'font-size:88%'>nHarnessing of myel= in cells may be possible.
<= span style=3D'font-size:88%'>nOlydendrocytes pro= vide myelin to alter cells.
<= span style=3D'font-size:88%'>nIt is not possible= to make long axons grow.
nInjured cord can p= romote neurogenesis by transpla= nted ESC derived neural precursors. nOptimizing the function of neuronal circuits is th= e current strategy.
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ES CE= LLS IN SPINAL REGENERATION
n
nES Cells
nReplicate indefinitely without aging
nAre pluripotent
nLikely to give rise to genetically normal cells
nCan easily be manipulated genetically.
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SOURCES OF ES CELLS
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nIn-vi= tro fertilization
nInner= cell mast from blastocyst can be flushed out prior to implantation.&#= 13;
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nCells= are often grown in culture.
nOne f= lask of cells gives rise to 625 flasks by the end of one week.
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PROBLEMS?
<= span style=3D'visibility:hidden'>n
nPluri= potent cells can deposit normal tissue in the wrong places. <= /span>
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nCan f= orm bone instead of muscle.
nAnswe= r:  transplant progenitors restricted= to making neural cells instead of unselected ES cells.
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INJEC= TION OF NEURAL PROGENITORS
<= span style=3D'visibility:hidden'>n
nMany d= ie others divide to fill the spinal defect.
<= span class=3DBB style=3D'position:absolute;left:-4.11%;top:.39em'>nThey = migrate away from the epicenter.
<= span class=3DBB style=3D'position:absolute;left:-3.88%;top:.39em'>nFollo= w radial glia along the central canal.
n60% b= ecame identifiable oligodendrocytes within two weeks.<= /div>
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RESULTS IN RATS
n
nTransplanted rat can:
nSupport body weight
nWalk – abnormally
nPartially lift tail
nSuggests partial remyelinization from oligodendrocytes.
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INTERPRETATION
<= span style=3D'visibility:hidden'>n
nRemye= linization in adult is much more difficult.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nES ce= ll remyelinization can occur.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nCures= are totally futuristic.
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nThere= is an ever increasing need.
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NEURO= GENESIS IN THE ADULT BRAIN
<= span style=3D'visibility:hidden'>n
nNew n= eurons are born in restricted regions of the adult brain. nCould stimulation of this process be applied to the treatment = of CNS disease?
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NEUROGENESIS
nIn the adult new glial cells derive from neural stem cells.
nHard to identify and isolate.
nFound in lateral ventricle and hippocampus.
nCells become electrically active.
nNeural progenitors have been identified.
nCompensated for dying neurons.
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CONTROL OF NEUROGENESIS
nCellu= lar – occurs close to blood vessels.
nMolec= ular – growth factors, hormones, neurotransmitters. nNeuro= genic stem cells can respond to injury. nLesion induced neurogenesis is inconsistent.
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STEM C= ELL CENTER AT TEXAS HEART INSTITUTE
n
nEnrolling patients into 3 studies.
nTreatment of:
nHeart failure
nMyocardial infarcts
nMyocardial ischemia
nIschemic legs
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HEART FAILURE TRIALS
n
nPatients:
nLow ejection fraction
nMaximal medical therapy
nExhausted conventional therapy
nBypass
nAngioplasty =
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n
nCurrently, no therapies in humans have ever been carried out using embryonic stem cells.
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OTHER POTENTIAL AREAS FOR RESEARCH
<= span style=3D'visibility:hidden'>n
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nCereb= ral Palsy
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nLeuke= mia
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nMulti= ple Sclerosis
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nParki= nson’s Disease
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nStrok= e
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PARKINSON’S DISEASE
n
nLima, Peru =
nStem cell implants – 47 patients
nAutologous stem cells infused into basal ganglie.
n75% of patients achieved >50% recovery at one month.
nThis recovery was maintained over time.
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MULTIPLE SCLEROSIS
nNice, France= 3;
nRussia
§Autologous hematopoietic in stem cell <= span style=3D'position:absolute;top:52.49%;left:12.73%;width:79.77%;height:6.0%= '>transplant
§High response rates and improved quality of life.
§They have restored the ability to walk.=
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ACUTE MYELOID LEUKEMIA
n
n
nBristol, England
§Two year old cured in 2007
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ONGOING STUDIES
<= span style=3D'font-size:88%'>nAstrocytoma
<= span style=3D'font-size:88%'>nRetinoblastoma = ;
<= span style=3D'font-size:88%'>nOvarian Cancer = ;
<= span style=3D'font-size:88%'>nSkin Cancer
<= span style=3D'font-size:88%'>nTesticular Cancer&= #13;
<= span style=3D'font-size:88%'>nLymphoma
<= span style=3D'font-size:88%'>nLeukemia
<= span style=3D'font-size:88%'>nMultiple myeloma
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ONGOING STUDIES
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nMyelo= dysplasia
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nBreast Cancer
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nNeuro= blastoma
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nRenal= Cell Carcinoma
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nSolid Tumors
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nSarco= mas
<= span class=3DBB style=3D'position:absolute;left:-4.44%;top:.39em'>nMyelo= fibrosis
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EXTENT OF CELL LOSS
<= span style=3D'visibility:hidden'>n
nNeuro= genesis is insufficient to induce functional recovery. nNeuro= genesis may not be capable of affection function. nCan t= hey differentiate into all the glial cell types?
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ES CELLS IN THE HEART
nUniversity of Texas
nMyocardial infarction
nmuscle death,
nfibrinous matrix
nInjection of ES cells into infarcted area. <= /div>
nCarried out percutaneously.
n
nDoes new functional muscle develop?
n
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CONCLUSIONS
n
<= span style=3D'font-size:88%'>nStem cell research one of the most exciting fields of modern medicine.
<= span style=3D'font-size:88%'>nHuge potential.
<= span style=3D'font-size:88%'>nIn its infancy.
<= span style=3D'font-size:88%'>nMechanism and control factors ill understood. =
<= span style=3D'font-size:88%'>nWe do know that ES cells can proliferate and are pluripotent.&= nbsp; We don’t know the disadvantages of pluropotency.
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SlideObj.style.display=3D"none" nObj =3D document.all.item("NotesObj") parent.SetHasNts(0) if( nObj ) {=20 nObj.style.display=3D"" parent.SetHasNts(1) } return 1 } function ChkAutoAdv() { if(SldHasTrans()) SlideObj.onfilterchange=3DAutoAdv else AutoAdv() } function AutoAdv() { if(!IsWin("PPTSld") || !gUseSldTimings )return var sld=3DGetCurSld() if( (sld.mAdvDelay>0) && !parent.IsFramesMode() ) setTimeout("parent.GoToNextSld()",sld.mAdvDelay) } function GetObj(id) { if(g_supportsPPTHTML) return document.all(id); else return document.getElementById(id); } function SldHasTrans() { return SlideObj.style.filter !=3D ""; } function GetSldId()=20 { var regExp =3D /file:\/\/\//i var pos =3D location.href.search(regExp) if (MHTMLPrefix !=3D "" && pos !=3D -1) sId =3D location.href.substring(pos) else { sId =3D RemoveFilePrefixFromHref(location.href); var regExp =3D /\// var fixedHref =3D sId var pos =3D -1 =09 pos =3D fixedHref.search(regExp) while (pos !=3D -1) { fixedHref =3D fixedHref.replace(regExp, "\\") pos =3D fixedHref.search(regExp) } =09 if (g_fBaseHyperlink =3D=3D true) sId =3D "file:///" + fixedHref; else sId =3D fixedHref.substring(fixedHref.lastIndexOf('\\') + 1) } =09 return sId } function HideMenu() { if( frames["PPTSld"] && PPTSld.document.all.item("ctx= tmenu") && PPTSld.ctxtmenu.style.display!=3D"none" ) { PPTSld.ctxtmenu.styl= e.display=3D'none'; return true } return false } function IsWin( name ) { return window.name =3D=3D name } function IsNts() { return IsWin("PPTNts") } function IsSldOrNts() { return( IsWin("PPTSld")||IsWin("PPTNts") ) } function SupportsPPTAnimation() { return( navigator.platform =3D=3D "Win32"= && navigator.appVersion.indexOf("Windows")>0 ) } function SupportsPPTHTML() { var appVer=3Dnavigator.appVersion, msie=3DappVer.indexOf("MSIE "), ver=3D0 if( msie >=3D 0 ) ver=3DparseFloat( appVer.substring( msie+5, appVer.indexOf(";",msie) ) ) else ver=3DparseInt(appVer) return( ver >=3D 4 && msie >=3D 0 ) } function _RSW() { if( !g_supportsPPTHTML || IsNts() || ( !g_scaleInFrame && (!IsWin("PPTSld") || !parent.IsFullScrMode()) ) ) return var padding=3D0; if( IsWin("PPTSld") && parent.IsFramesMode() ) padding=3D6 cltWidth=3Ddocument.body.clientWidth-padding cltHeight=3Ddocument.body.clientHeight-padding factor=3D(1.0*cltWidth)/g_origW if( cltHeight < g_origH*factor ) factor=3D(1.0*cltHeight)/g_origH newSize =3D g_origSz * factor if( newSize < 1 ) newSize=3D1 s=3DSlideObj.style s.fontSize=3DnewSize+"px" s.posWidth=3Dg_origW*factor s.posHeight=3Dg_origH*factor s.posLeft=3D(cltWidth-s.posWidth+padding)/2 s.posTop=3D(cltHeight-s.posHeight+padding)/2 if( g_scaleHyperlinks ) ScaleHyperlinks( factor ) } function _InitAnimations() { animRuntimeInstalled =3D ''+document.body.localTime !=3D 'undefined'; isFullScreen =3D (window.name =3D=3D "PPTSld") && !parent.IsFramesMode(); g_animUseRuntime =3D g_showAnimation && animRuntimeInstalled && !(isFullSc= reen && parent.IsSldVisited()); if( g_animUseRuntime ) { collSeq =3D document.all.tags("seq"); if( collSeq !=3D null ) { for(ii=3D0;ii numSlds ) gSldJumpIdx =3D numSlds; if ( gSldJumpIdx >=3D 0 ) { if ( gSldJumpIdx =3D=3D 0 ) gSldJumpIdx =3D 1; var jumpTo =3D parseInt(gSldJumpIdx); gSldJump =3D 0; gSldJumpIdx =3D ""; win.GoToSld( parent.GetSldList().mList[jumpTo-1].mSldHref ) } } } function _KDH() { if( event.keyCode =3D=3D 8 ) { event.returnValue =3D 0; parent.GoToPrevSld(); } } function PlaySound( href, loop ){ href=3D unescape(href); if( window.event ) window.event.cancelBubble =3D true; pptSound.src =3D href; pptSound.loop =3D loop ? -1 : 0; } function DocumentOnClick() { if( IsNts() || parent.HideMenu() ) return; if( ( g_allowAdvOnClick && (window.name=3D=3D"PPTSld") && !parent.IsFrames= Mode() ) || (event && event.keyCode=3D=3D32) ) { =09 if( g_animUseRuntime && g_animMainSequence && g_animMainSequence.cangonex= t ) return; parent.GoToNextSld(); } } var g_supportsPPTHTML =3D SupportsPPTHTML(), g_scaleInFrame =3D 1, gId=3D""= , g_bgSound=3D"", g_scaleHyperlinks =3D false, g_allowAdvOnClick =3D 1, g_showInBrowser = =3D 0, gLoopCont =3D 0, gUseSldTimings =3D 1; var g_showAnimation =3D g_supportsPPTHTML && SupportsPPTAnimation() && ( (w= indow.name=3D=3D"PPTSld" && !parent.IsFramesMode()) || g_showInBrowser );va= r g_animManager =3D null; var g_animUseRuntime =3D false; var g_animItemsToHide, g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"End of slide show, click to exit.", SCREEN_MODE=3D"Fram= es", gIsEndShow=3D0, NUM_VIS_SLDS=3D53, SCRIPT_HREF=3D"script.js", FULLSCR_= HREF=3D"fullscreen.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var g_fBaseHyperlink =3D false; var gMainDoc=3Dnew Array(new hrefList("slide0001.htm",1,-1,1),new hrefList(= "slide0002.htm",1,-1,1),new hrefList("slide0003.htm",1,-1,1),new hrefList("= slide0004.htm",1,-1,1),new hrefList("slide0005.htm",1,-1,1),new hrefList("s= lide0006.htm",1,-1,1),new hrefList("slide0007.htm",1,-1,1),new hrefList("sl= ide0008.htm",1,-1,1),new hrefList("slide0009.htm",1,-1,1),new hrefList("sli= de0010.htm",1,-1,1),new hrefList("slide0011.htm",1,-1,1),new hrefList("slid= e0012.htm",1,-1,1),new hrefList("slide0013.htm",1,-1,1),new hrefList("slide= 0014.htm",1,-1,1),new hrefList("slide0015.htm",1,-1,1),new hrefList("slide0= 016.htm",1,-1,1),new hrefList("slide0017.htm",1,-1,1),new hrefList("slide00= 18.htm",1,-1,1),new hrefList("slide0019.htm",1,-1,1),new hrefList("slide002= 0.htm",1,-1,1),new hrefList("slide0021.htm",1,-1,1),new hrefList("slide0022= .htm",1,-1,1),new hrefList("slide0023.htm",1,-1,1),new hrefList("slide0024.= htm",1,-1,1),new hrefList("slide0025.htm",1,-1,1),new hrefList("slide0026.h= tm",1,-1,1),new hrefList("slide0027.htm",1,-1,1),new hrefList("slide0028.ht= m",1,-1,1),new hrefList("slide0029.htm",1,-1,1),new hrefList("slide0030.htm= ",1,-1,1),new hrefList("slide0031.htm",1,-1,1),new hrefList("slide0032.htm"= ,1,-1,1),new hrefList("slide0033.htm",1,-1,1),new hrefList("slide0034.htm",= 1,-1,1),new hrefList("slide0035.htm",1,-1,1),new hrefList("slide0036.htm",1= ,-1,1),new hrefList("slide0037.htm",1,-1,1),new hrefList("slide0038.htm",1,= -1,1),new hrefList("slide0039.htm",1,-1,1),new hrefList("slide0040.htm",1,-= 1,1),new hrefList("slide0041.htm",1,-1,1),new hrefList("slide0042.htm",1,-1= ,1),new hrefList("slide0043.htm",1,-1,1),new hrefList("slide0044.htm",1,-1,= 1),new hrefList("slide0045.htm",1,-1,1),new hrefList("slide0046.htm",1,-1,1= ),new hrefList("slide0047.htm",1,-1,1),new hrefList("slide0048.htm",1,-1,1)= ,new hrefList("slide0049.htm",1,-1,1),new hrefList("slide0050.htm",1,-1,1),= new hrefList("slide0051.htm",1,-1,1),new hrefList("slide0052.htm",1,-1,1),n= ew hrefList("slide0053.htm",1,-1,1)); /********************************************* Frameset functions These functions control slide navigation and state of the frameset. **********************************************/ function RemoveFilePrefixFromHref(href) { var regExp =3D /^file:\/\/\//i; return href.replace(regExp, "") } function FullScrInit() { g_allowAdvOnClick =3D GetCurSld().mAdvOnClk document.body.style.backgroundColor=3D"black" document.oncontextmenu=3Dparent._CM; document.onkeydown =3D _KDH; document.ondragstart=3DCancel document.onselectstart=3DCancel self.focus() } function Redirect( frmId ) {=09 var str=3Ddocument.location.hash,idx=3Dstr.indexOf('#'), sId=3DGetSldId() if(idx>=3D0) str=3Dstr.substr(1); if( window.name !=3D frmId && ( sId !=3D str) ) { obj =3D GetObj("Main-File") window.location.href=3Dobj.href+"#"+sId return 1 } return 0 } var MHTMLPrefix =3D CalculateMHTMLPrefix();=20 function CalculateMHTMLPrefix() { if ( document.location.protocol =3D=3D 'mhtml:') {=20 href=3Dnew String(document.location.href)=20 Start=3Dhref.indexOf('!')+1=20 End=3Dhref.lastIndexOf('/')+1=20 if (End < Start)=20 return href.substring(0, Start)=20 else=20 return href.substring(0, End)=20 } return ''; } function GetTags(base,tag) { if(g_supportsPPTHTML) return base.all.tags(tag); else return base.getElementsByTagName(tag); } function UpdNtsPane(){ if(frames["PPTNts"]) PPTNts.location.replace( MHTMLP= refix+GetHrefObj( gCurSld ).mNtsHref ) } function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() } function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() } function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() } function SetHasNts( fVal ) { if( gHasNts !=3D fVal ) { gHasNts=3DfVal UpdNavPane() } } function ToggleOtlText() { gOtlTxtExp=3D!gOtlTxtExp UpdOtlPane() } function ClearMedia() { // Clear any sounds playing before launching another browser window. Other= wise, // in fullscreen mode, you'll continue to hear the sound in the frames mod= e. if (PPTSld.pptSound) PPTSld.pptSound.loop =3D 0; } function FullScreen() {=20 if ( PPTSld.g_animUseRuntime ) PPTSld.document.body.pause(); ClearMedia(); var href =3D ( document.location.protocol =3D=3D 'mhtml:') ? FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if (MHTMLPrefix !=3D "") href =3D RemoveFilePrefixFromHref(href) if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { =20 obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else =20 return gCurSld } function GetNumSlds() { =20 if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; =09 if (MHTMLPrefix !=3D "") PPTSld.location.replace(MHTMLPrefix+RemoveFilePrefixFromHref(obj.mSldHref= )) else PPTSld.location.replace(obj.mSldHref) =09 if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 if (MHTMLPrefix !=3D "") PPTSld.location.replace(MHTMLPrefix+RemoveFilePrefixFromHref(href)) else PPTSld.location.replace(href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; =09 var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document var dir =3D doc.body.dir if( dir !=3D "rtl" ) dir =3D "ltr"; doc.open() doc.writeln('


' + ENDSHOW_MESG + '

') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); /********************************************* Navigation button implementation There are 2 types of buttons: ImgBtn, TxtBtn implemented as function objects. They share a similiar interface so the event handlers can call SetActive, for example, on a button=20 object without needing to know exactly=20 what type of button it is. **********************************************/ //---------------------------------- function ImgBtn( oId,bId,w,action ) //---------------------------------- { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0=20 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { =20 obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0=20 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } =20 } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } //----------------------------------------- function TxtBtn( oId,oeId,action,chkState ) //----------------------------------------- { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { =20 this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) {=20 if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } =20 } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1=20 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() {=20 b =3D gBtnArr[this.mBorderId]=20 if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() {=20 b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState /********************************************* Context menu implementation _CM() is the function that's hooked up to the oncontextmenu event. Once we're asked to show the menu, we first build it by creating DIVs on-the-fly. Then we position it=20 within the screen area so it doesn't get clipped. Creating the DIVs using createElement() means we don't have to write out any extra HTML into the slide HTML files. **********************************************/ var sNext=3D"Next",sPrev=3D"Previous",sEnd=3D"End Show",sFont=3D"Arial",sAr= row=3D"Arrow",sFreeform=3D"Freeform",sRect=3D"Rectangle",sOval=3D"Oval" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) =09 CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); else GoToNextSld() } function M_GoPrevSld() { if( gIsEndShow ) { gIsEndShow=3D0; history.back();= PPTSld.event.cancelBubble=3Dtrue; } else GoToPrevSld() } function M_True() { return true } function M_End() { window.close( self ) } function CreateMenuItem( node,text,action,eval ) { var e=3DCreateItem( node ) e.type=3D"menuitem" e.Action=3Daction e.IsActive=3Deval e.innerHTML=3Dtext if( !e.IsActive() ) e.style.color=3D"threedshadow" e.onclick=3DPerform e.onmouseover=3DHighlight e.onmouseout=3DDeselect s=3De.style; s.fontFamily=3DsFont s.fontSize=3D"9pt" s.paddingLeft=3D2 } function CreateSeparator( node ) { var sObj=3DCreateItem( node ) SetCMBorder(sObj,"menu","menu") var s=3DsObj.style s.borderTopColor=3D"threedshadow" s.borderBottomColor=3D"threedhighlight" s.height=3D1 s.fontSize=3D"0px" } function CreateItem( node ) { var elem=3DPPTSld.document.createElement("DIV") node.insertBefore( elem ) return elem } function SetCMBorder( o,ltClr,rbClr ) { var s=3Do.style s.backgroundColor=3D"menu" s.borderStyle=3D"solid" s.borderWidth=3D1 s.borderColor=3DltClr+" "+rbClr+" "+rbClr+" "+ltClr } ------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/fullscreen.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" ------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/buttons.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhWAESAPf4AAAAAIAAAACAAICAAAAAgIAAgACAgICAgAQEBISEBASEBISEhAQEhMTExAQE /KTM9Pz8/ERERPz8BAT8/KSkpGRkhMTcxCRkxAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAMDAwP8AAAD/AP//AAAA//8A/wD//////yH5BAEAAPgALAAAAABYARIA QAj/APEJHEiwoMGDCBMqXMiwocOHECNKnEixosWLGCEiQKCRo8YDIEHig2DxAD4LKD1avDDhgUuV GUOGbHBR5QGYFBG4fHAB58EKECJEwCfUYAWPFGAygLCRKQIIUKM6WAohKUGgCikcFWh1INWoYKVS 7SrQ41GbIhGYLAuVQVOoC+IyXUuUAkG1HE3KFDhAwkgJc5/qDZmxsOHDiBMrXsy4sePDGyP7lChT 5MULmDPnlDw5YmW6FTNrfgi0aNGEZPEtbfo2bNTUpYeeRph69dPWrqvCNFuBIGG1A59CYPAVQtwF cw0OxWdTIOiBCQAjaAB17fOFII8voGmRc+eOkilm/z9OvTtnjN4hYk1YoT3H2rnBOphPAB9skgq3 2lcaX/5YnJVt9BloAar12UEIRGCZdQghMABI1Tn32IQUVthYexhmqGGGMBHAgIfEhSiiiATAtOGJ GnYI4ogsEldicAYOGNJGZaml3Y0L3NTZUBz1xlB0PUoUI0jlVWRSVN89xBKSGB1YU1TMoRfVBEle hR9HQs1GEHxQ3RZZbveZthxt/LEmXHyp5XWABSCpaRJwbM13gAACPHVccgJFgF+NEhqUAEkQSCfc ddhVhh5dVTaEAEsTUNlkZUWKN9BNGDE6QU8OrSdbllzpxyVTTrU21Uj3scfhp7nNpyoDaTIEJ3P9 If8J2kbLNYegg9JRBwGhFvbq66+ZUiDssMQWK2yHxiZLrInKNotss8l+B5yBrr5p2UIJvortANoC 6+234IbLla/elRtZjeamh5CC3Sb0YLvixivvYkDFGlZqBNh7r4n6goVvv6/ZlCicN+kL71ALTnpQ dCIZp7CihkLA3UQmoWRBogyxtBPGCTnZncVR1rSTo5nuydC/Eo8kUH+lOoTyyvjoCuZu+PjYIKLD rZybtrM1d10C0QXKEVz48GrQkEeWdFJKlbb0UmEeV0SzyC6RvNB646LWIVjUNeC1zAFb+RDKXksM 9r531bwmpQW9+hQDDsx5pqwDRWBXcFES2hc+gEn/wJGdEEWdE6LoWcoxQweaTNmkhydkKaYMYY2Q p1unbLl/pPKbn3v7EZRvrKvqlja2ONvrtoIw9tn2g32/tQBlhtYkU+MNikZ7QYlPDPuMldpOmuJX nVo5VF3HjI+q89mteX5IDd/fVFClma5kiE4f2XUJhlz09gj2NbTDRifkmu7zXjR++RVJ/lPznuvr QH2iD6S+UewP9Dnon6d2NLwN8n90BB6hC68cFMCHoe+ACASP9c4DowUyEF0OPFcDI0ij/VXPaAKC UfhS95AKGoQ7IBRICI1HwhGaUIQoLGEKT6jCFrLwhSuMoQtlCMMZ2rCGOKShDm+4wxzy8Ic+DGIP B4cIRCKGMCAAOy== ------=_NextPart_01CAE6B5.14BF9790 Content-Location: file:///C:/2359C650/Taylor_GR_4-21_StemCells.ppt_042010_files/frame.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; 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Outline
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