Specimen Collection

Pinworm Test

Pinworm (Enterobius vermicularis) detection

A. The specimen is collected from the skin of the perianal area first thing in the morning, before the patient has bathed or used the toilet.

B. To obtain a sample from the perianal area, take a piece of clear cellulose (Scotch) tape and press the tape firmly several times against the right and left perianal folds.

C. Place the clear cellulose tape (adhesive side down) on a microscope slide. Tear the tape even with the end of the slide.

D. Label with patient name and date.

E. Submit the tapes and slides to the laboratory in a plastic bag.

F. Preparations should be taken for at least 4 to 6 consecutive days with negative results before a patient is considered free of the infection.


A. Pinworm eggs are usually infectious. The use of glass slides and tapes may expose you to these eggs.

B. Some recommend the use of the Swube (paddle with sticky adhesive coat; (Becton Dickinson) as a safer alternative.