Szczesny Research Lab

Publications

 

 

47. Koo SJ, Szczesny B, Wan X, Putluri N, Garg NJ. (2018) Pentose Phosphate Shunt Modulates Reactive Oxygen Species and Nitric Oxide Production Controlling Trypanosoma cruzi in Macrophages. Front Immunol. 9:202. https://www.ncbi.nlm.nih.gov/pubmed/29503646

46. Szczesny B, Marcatti M, Ahmad A, Montalbano M, Brunyánszki A, Bibli SI, Papapetropoulos A, Szabo C. (2018) Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells. Sci Rep. 8(1):914. https://www.ncbi.nlm.nih.gov/pubmed/29343810

45. Ibitokou SA, Dillon BE, Sinha M, Szczesny B, Delgadillo A, Reda Abdelrahman D, Szabo C, Abu-Elheiga L, Porter C, Tuvdendorj D, Stephens R. (2018) Early Inhibition of Fatty Acid Synthesis Reduces Generation of Memory Precursor Effector T Cells in Chronic Infection. J Immunol. 200(2):643-656. https://www.ncbi.nlm.nih.gov/pubmed/29237780

44. Oláh G, Módis K, Törö G, Hellmich MR, Szczesny B, Szabo C. (2017) Role of endogenous and exogenous nitric oxide, carbon monoxide and hydrogen sulfide in HCT116 colon cancer cell proliferation. Biochem Pharmacol. 149: 186-204. https://www.ncbi.nlm.nih.gov/pubmed/29074106

43. Phillips CM, Zatarain JR, Nicholls ME, Porter C, Widen SG, Thanki K, Johnson P, Jawad MU, Moyer MP, Randall JW, Hellmich JL, Maskey M, Qiu S, Wood TG, Druzhyna N, Szczesny B, Módis K, Szabo C, Chao C, Hellmich MR. (2017) Upregulation of Cystathionine-β-Synthase in Colonic Epithelia Reprograms Metabolism and Promotes Carcinogenesis. Cancer Res. 77(21):5741-5754. https://www.ncbi.nlm.nih.gov/pubmed/28923859

42. López-García I, Gerő D, Szczesny B, Szoleczky P, Olah G, Módis K, Zhang K, Gao J, Wu P, Sowers LC, DeWitt D, Prough DS, Szabo C. (2018) Development of a stretch-induced neurotrauma model for medium-throughput screening in vitro: identification of rifampicin as a neuroprotectant. Br J Pharmacol. 175(21):284-300. https://www.ncbi.nlm.nih.gov/pubmed/27723079

41. Korkmaz-Icöz S*, Szczesny B*, Marcatti M, Li S, Ruppert M, Lasitschka F, Loganathan S, Szabó C, Szabó G. (2017) Olaparib protects cardiomyocytes against oxidative stress and improves graft contractility during the early phase after heart transplantation in rats. Br J Pharmacol 175(2):246-261 *Both authors contributed equally to the publication https://www.ncbi.nlm.nih.gov/pubmed/28806493

40. Steiger AK, Marcatti M, Szabo C, Szczesny B, Pluth MD. (2017) Inhibition of Mitochondrial Bioenergetics by Esterase-Triggered COS/H2S Donors. ACS Chem Biol. 12(8): 2117-2123. https://www.ncbi.nlm.nih.gov/pubmed/28613823

39. Szczesny B, Marcatti M, Zatarain JR, Druzhyna N, Wiktorowicz JE, Nagy P, Hellmich MR, Szabo C. (2016) Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics. Sci Rep. 6:36125. https://www.ncbi.nlm.nih.gov/pubmed/27808278

38. Koo SJ, Chowdhury IH, Szczesny B, Wan X, Garg NJ. (2016) Macrophages Promote Oxidative Metabolism To Drive Nitric Oxide Generation in Response to Trypanosoma cruzi. Infect Immun. 84(12):3527-3541. https://www.ncbi.nlm.nih.gov/pubmed/27698021

37. Druzhyna N, Szczesny B, Olah G, Módis K, Asimakopoulou A, Pavlidou A, Szoleczky P, Gerö D, Yanagi K, Törö G, López-García I, Myrianthopoulos V, Mikros E, Zatarain JR, Chao C, Papapetropoulos A, Hellmich MR, Szabo C. (2016) Screening of a composite library of clinically used drugs and well-characterized pharmacological compounds for cystathionine β-synthase inhibition identifies benserazide as a drug potentially suitable for repurposing for the experimental therapy of colon cancer. Pharmacol Res. 113(Pt A):18-37. https://www.ncbi.nlm.nih.gov/pubmed/27521834

36. Chao C, Zatarain JR, Ding Y, Coletta C, Mrazek AA, Druzhyna N, Johnson P, Chen H, Hellmich JL, Asimakopoulou A, Yanagi K, Olah G, Szoleczky P, Törö G, Bohanon FJ, Cheema M, Lewis R, Eckelbarger D, Ahmad A, Módis K, Untereiner A, Szczesny B, 35. Papapetropoulos A, Zhou J, Hellmich MR, Szabo C. (2016) Cystathionine-beta-synthase inhibition for colon cancer: Enhancement of the efficacy of aminooxyacetic acid via the prodrug approach. Mol Med. 22:361-379 https://www.ncbi.nlm.nih.gov/pubmed/27257787

34. Brunyanszki A, Szczesny B, Virág L and Szabo C. (2016) Mitochondrial Poly (ADP-ribose) Polymerase: The Wizard of Oz at Work. Free Radic Biol Med, 100:257-270. https://www.ncbi.nlm.nih.gov/pubmed/26964508

33. Porter C, Herndon DN, Bhattarai N, Ogunbileje JO, Szczesny B, Szabo C, Toliver-Kinsky T and Sidossis LS. (2016) Differential acute and chronic effects of burn trauma on murine skeletal muscle bioenergetics. Burns. 42(1): 112-122. https://www.ncbi.nlm.nih.gov/pubmed/26615714

32. Rios EC, Soriano FG, Olah G, Gerö D, Szczesny B, Szabo C. (2016) Hydrogen sulfide modulates chromatin remodeling and inflammatory mediator production in response to endotoxin, but does not play a role in the development of endotoxin tolerance. J Inflamm (Lond) 13:10. https://www.ncbi.nlm.nih.gov/pubmed/27042162

31. Ahmad A, Olah G, Szczesny B, Wood ME, Whiteman M, Szabo C. (2016) AP39, A Mitochondrially- Targeted Hydrogen Sulfide Donor, Exerts Protective Effects in Renal Epithelial Cells Subjected to Oxidative Stress in Vitro and in Acute Renal Injury in Vivo. Shock. 45(1): 88-97. https://www.ncbi.nlm.nih.gov/pubmed/26513708

30. Szczesny B, Brunyánszki A, Ahmad A, Oláh G, Porter C, Toliver-Kinsky T, Sidossis L, Herndon DN and Szabo C. (2015) Time-dependent and organ-specific changes in mitochondrial function, mitochondrial DNA integrity, oxidative stress and mononuclear cell infiltration in a mouse model of burn injury. PLoS One. 10(12): e0143730. https://www.ncbi.nlm.nih.gov/pubmed/26630679

29. Brunyanszki A, Erdelyi K, Szczesny B, Olah G, Salomao R, Herndon DN, Szabo C. (2015) Upregulation and mitochondrial sequestration of hemoglobins occurs in circulating leukocytes during critical illness, conferring a cytoprotective phenotype. Mol Med. 21(1):666-675. https://www.ncbi.nlm.nih.gov/pubmed/26322851

28. Olah G, Szczesny B, Brunyanszki A, Lopez-Garcia I, Gero D, Radak Z and Szabo C. (2015) Differentiation-associated downregulation of poly(ADP-ribose) polymerase-1 expression in myoblasts serves to increase their oxidative stress resistance. PLoS One. 10(7): e0134227 https://www.ncbi.nlm.nih.gov/pubmed/26218895

27. Budworth H, Harris FR, Williams P, Lee DY, Holt A, Pahnke J, Szczesny B, Acevedo-Torres K, Ayala-Peña S, McMurray CT (2015) Suppression of somatic expansion delays the onset of pathophysiology in a mouse model of Huntington’s disease. PLoS Genetics. 11(8): e1005267 https://www.ncbi.nlm.nih.gov/pubmed/26247199

26. Porter C, Herndon DN, Bhattarai N, Ogunbileje JO, Szczesny B, Szabo C, Toliver-Kinsky T, Sidossis LS. (2015) Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue. Shock. 44(3): 258-64. https://www.ncbi.nlm.nih.gov/pubmed/26009824

25. Rios EC, Szczesny B, Soriano FG, Olah G, Szabo C. (2015) Hydrogen sulfide attenuates cytokine production through the modulation of chromatin remodeling. Int J Mol Med. 35(6): 1741-6. https://www.ncbi.nlm.nih.gov/pubmed/25873160

24. Coletta C, Módis K, Szczesny B, Brunyánszki A, Oláh G, Rios EC, Yanagi K, Ahmad A, Papapetropoulos A, Szabo C (2015) Regulation of Vascular Tone, Angiogenesis and Cellular Bioenergetics by the 3-Mercaptopyruvate Sulfurtransferase/H2S Pathway: Functional Impairment by Hyperglycemia and Restoration by -α-Lipoic Acid. Mol Med. 21: 1-14. https://www.ncbi.nlm.nih.gov/pubmed/25715337

23. Szczesny B, Brunyanszki A, Olah G, Mitra S, Szabo C. (2014) Opposing roles of mitochondrial and nuclear PARP1 in the regulation of mitochondrial and nuclear DNA integrity: implications for the regulation of mitochondrial function. Nucleic Acids Res. 42(21): 13161-73. https://www.ncbi.nlm.nih.gov/pubmed/25378300

22. Brunyanszki A, Olah G, Coletta C, Szczesny B, Szabo C. (2014) Regulation of mitochondrial poly(ADP-ribose) polymerase activation by the b-adrenoceptor/cAMP/proteinase kinase A axis during oxidative stress. Mol Pharmacol. 86(4): 450-62. https://www.ncbi.nlm.nih.gov/pubmed/25069723

21. Szczesny B, Módis K, Yanagi K, Coletta C, Le Trionnaire S, Wood ME, Whiteman M, Szabo C. (2014) AP39, a hydrogen sulfide donor, stimulates cellular bioenergetics, exerts cytoprotective effects and protects against the loss of mitochondrial DNA integrity in oxidatively stressed endothelial cells in vitro. Nitric Oxide. 41: 120-30. https://www.ncbi.nlm.nih.gov/pubmed/24755204

20. Módis K, Coletta C, Asimakopoulou A, Szczesny B, Chao C, Papapetropoulos A, Hellmich MR3, Szabo C (2014) Effect of S-adenosyl-l-methionine (SAM), an allosteric activator of cystathionine-β-synthase (CBS) on colorectal cancer cell proliferation and bioenergetics in vitro. Nitric Oxide. 41: 146-56. https://www.ncbi.nlm.nih.gov/pubmed/24667534

19. Trionnaire SL, Perry A, Szczesny B, Szabo C, Winyard PG, Whatmore JL, Wood ME and Whiteman M. (2014) The synthesis and functional evaluation of a mitochondria-targeted hydrogen sulfide donor, (10-oxo-10-(4-(3-thioxo-3H-1,2-dithiol-5-yl)phenoxy) decyl) triphenylphosphonium bromide (AP39) Med. Chem. Commun. 5: 728-736. https://pubs.rsc.org/en/content/articlelanding/2014/md/c3md00323j - !divAbstract

18. Szczesny B, Olah G, Walker DK, Volpi E, Rasmussen BB, Szabo C and Mitra S. (2013) Deficiency in Repair of the Mitochondrial Genome Sensitizes Proliferating C2C12 Myoblasts to Oxidative Damage. PLoS One 8(9): e75201. https://www.ncbi.nlm.nih.gov/pubmed/24066171

17. Szabo C, Coletta C, Chao C, Modis K, Szczesny B, Papapetropoulus A and Hellmich MR (2013) Tumor-derived H2S, produced by cystathionine-b-synthase, stimulates bioenergetics, cell proliferation and angiogenesis in colon cancer. Proc Natl Acad Sci USA 110(30): 12474-9. https://www.ncbi.nlm.nih.gov/pubmed/23836652

16. Tsutakawa SE, Shin DS, Mol CD, Izumi T, Arvai AS, Mantha AK, Szczesny B, Ivanov IN, Hosfield DJ, Pique ME, Frankel KA, Hitomi K, Cunningham RP, Mitra S, Tainer JA. (2013) Conserved structural chemistry for incision activity in structurally non-homologous APE1 and Endonuclease IV DNA repair enzymes. J. Biol. Chem. 288(12): 8445-55. https://www.ncbi.nlm.nih.gov/pubmed/23355472

15. Bacsi A, Aguilera-Aguirre L, Szczesny B, Radak Z, Hazra TK, Sur S, Ba X and Boldogh I. (2013) Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation. DNA Repair 12(1): 18-26. https://www.ncbi.nlm.nih.gov/pubmed/23127499

14. Hegde ML, Mantha AK, Bhakat, KK, Hazra TK, Mitra S and Szczesny B. (2012) Oxidative genome damage and its repair: implications in aging and neurodegenerative diseases. Mech Ageing Dev. 133(4): 157-68. https://www.ncbi.nlm.nih.gov/pubmed/22313689

13. Mandal SM, Hegde ML, Hegde PM, Banerjee D, Szczesny B, Boldogh I, Falkenberg M, Gustafsson CM, Sarkar PS, and Hazra TK. (2012) Role of human DNA glycosylase Nei-like 2 (NEIL2) and single strand break repair protein polynucleotide kinase 3'-phosphatase in maintenance of mitochondrial genome. J. Biol. Chem. 287(4): 2819-29. https://www.ncbi.nlm.nih.gov/pubmed/22130663

12. Tann AW, Boldogh I, Meiss G, Qian W, van Houten B, Mitra S and Szczesny B. (2011) Apoptosis induced by single-strand breaks in the mitochondrial genome: critical role of mitochondrial 5’ exo/endonuclease, EXOG. J. Biol. Chem. 286(3): 1975-83. https://www.ncbi.nlm.nih.gov/pubmed/21768646

11. Zhang H, Xie C, Spencer HJ, Zuo C, Higuchi M, Ranganathan G, Kern PA, Chou MW, Huang Q, Szczesny B, Mitra S, Watson AJ, Margison GP and Fan CY. (2011) Obesity and hepatosteatosis in mice with enhanced oxidative DNA damage processing in mitochondria. Am J Pathol. 178(4): 1715-27. https://www.ncbi.nlm.nih.gov/pubmed/21435453

10. Kieper J, Lauber C, Gimadutdinow O, Urbańska A, Cymerman I, Ghosh M, Szczesny B and Meiss G. (2010) Production and characterization of recombinant protein preparations of Endonuclease G-homologs from yeast, C. elegans and humans. Protein Expression and Purification. 73(1): 99-106. https://www.ncbi.nlm.nih.gov/pubmed/20382228

9. Szczesny B, Tann AW and Mitra S. (2010) Age- and tissue-specific changes in mitochondrial and nuclear DNA base excision repair activity in mice: Susceptibility of skeletal muscles to oxidative injury. Mech Ageing Dev. 131(5): 330-337. https://www.ncbi.nlm.nih.gov/pubmed/20363243

8. Szczesny B, Tann AW, Longley MJ, Copeland WC and Mitra, S. (2008) Long patch base excision repair in mammalian mitochondrial genomes. J. Biol. Chem. 283(39): 26349-56. https://www.ncbi.nlm.nih.gov/pubmed/18635552

7. Mitra S, Izumi T, Boldogh I, Bhakat KK, Chattopadhyay R and Szczesny B. (2007) Intracellular trafficking and regulation of mammalian AP-endonuclease I (APE1), an essential DNA repair protein. DNA Repair 6(4): 461-469. https://www.ncbi.nlm.nih.gov/pubmed/17166779

6. Chattopadhyay R, Widerhold L, Szczesny B, Izumi T, Hazra TK and Mitra, S. (2006) Identification and characterization of mitochondrial AP-endonuclease in mammalian cells. Nucleic Acids Res. 34(7): 2067-2076. https://www.ncbi.nlm.nih.gov/pubmed/16617147

5. Szczesny B and Mitra S. (2005) Age-dependent changes in intracellular distribution of AP endonuclease activity. Mech Ageing Dev. 126(10): 1071-1078. https://www.ncbi.nlm.nih.gov/pubmed/15951004

4. Szczesny B, Bhakat KK, Mitra S and Boldogh I. (2004) Age-dependent modulation of DNA repair enzymes by covalent modification and subcellular distribution. Mech Ageing Dev. 125(10-11): 755-765. https://www.ncbi.nlm.nih.gov/pubmed/15541770

3. Gibala M*, Szczesny B*, Kieleczawa J and Janska H. (2004) The pea mitochondrial atp6: RNA editing and similarity of presequences in the Vicieae tribe. Curr Genet. 46(4): 235-239. *Both authors contributed equally to the publication. https://www.ncbi.nlm.nih.gov/pubmed/15322816

2. Szczesny B, Hazra TK, Papaconstantinou J, Mitra S, Boldogh I. (2003) Age-dependent deficiency in import of mitochondrial DNA glycosylase required for repair of oxidatively damage bases. Proc Natl Acad Sci USA 100(19): 10670-10675. https://www.ncbi.nlm.nih.gov/pubmed/12960370

1. Kolodziejczak M, Kolaczkowska A, Szczesny B, Urantowka A, Knorpp C, Kieleczawa J, Janska H. (2002) A higher plant mitochondrial homologue of the yeast m-AAA protease. Molecular cloning, localization, and putative function. J. Biol. Chem. 277(46): 43792-437928. https://www.ncbi.nlm.nih.gov/pubmed/12228240