UTMB Biomedical Research Facility

Protein Synthesis and Biomarker Core

Mission Statement

In close collaboration with investigators, the Protein Synthesis and Biomarker Core provides services for the production and purification of bacterial and yeast proteins. A wide variety of chromatographic, and other purification methods are available to obtain a high level of protein purity suitable for protein structural studies as well as other applications. Protein homogeneity (quality control) is routinely assessed by SDS-PAGE and mass spectrometry. Other evaluation methods are also available upon request.

Services Available

  • Biosynthesis of recombinant proteins in 5L or 20L bacterial or yeast fermentations
  • Protein Purification
    • Ion exchange
    • Gel permeation
    • Isoelectric focusing
    • Immunoaffinity
    • His-tag affinity
    • Hydrophobic interaction
  • Collaborative protein synthesis/purification methods development
  • Polyclonal antibody production and purification
    • Peptide conjugation to KLH and purification
    • Antiserum production (out sourced)
    • Monospecific antibody affinity purification
  • Data analysis (Biomarker Computational Module)

Quality Control

  • 1D & 2D gel electrophoresis (Separation Technology Core)
  • Totallab (formerly Nonlinear Dynamics) SameSpots (Separation Technology Core
  • Western blot analysis
  • MALDI or ESI mass analysis (Mass Spectrometry Core)
  • Edman protein sequencing (Protein Chemistry Core)
  • Compositional amino acid analysis (Protein Chemistry Core)
  • Carboxypeptidase C-terminal analysis (Protein Chemistry Core)

Major Equipment

  • 5- and 20-L New Brunswick fermentors
  • French press with the rapid refill 40 ml cell for large quantity cell lysis
  • Continuous flow concentrator for the recovery of secreted proteins from cell cultures
  • Beckman L70 Ultra Centrifuges with type 19 ultracentrifuge rotor with up to 1 L processing capacity
  • Pharmacia AKTA Explorer micro and preparative scale work station for medium and high pressure column chromatography
  • Other chromatography systems with a wide selection of support columns
  • Pharmacia AKTA FPLC
  • Invitrogen ZOOM® IEF Fractionator for preparative IEF
  • Bio-Rad Rotofor apparatus for preparative IEF