Transgenic Mouse Core Facility
The Transgenic Mouse Core Facility (TMCF)
is committed to providing customized, flexible, and comprehensive
services to UTMB investigators utilizing genetically engineered mouse
models. Its services include: generation of
transgenic mice, gene targeting, cryopreservation of mouse embryos and
sperm, rederivation, breeding, and genotyping. It can also offer
consultations and assistance with grant/animal protocol writing. The TMCF is located on the ground floor of the Medical Research Building, inside the Animal Resource Center.
To provide comprehensive and flexible support to investigators
engaging in genetically engineered mouse model research, and to
facilitate the growth of the mouse research and translational research at UTMB.
Generation of Transgenic Mice
Generation of transgenic mice by microinjection of DNA into the pronucleus of a fertilized mouse oocyte
Genetic engineering of mouse embryonic stem cells, and
microinjection of the cells into pre-implantation-stage mouse embryos.
A. Cryopreservation of Mouse Embryos
For each day of cryopreservation requested, we will isolate
and cryopreserve 8-cell embryos from as many as fifteen super-ovulated
female mice using the slow-cooling method.
B. Cryopreservation of Mouse Sperm
We will cryopreserve sperm from two male mice using the
Jackson Lab Sperm Cryopreservation Kit. This service includes an
assessment for the ability of cryopreserved sperm to fertilize oocytes
when thawed, and three-year storage of the sperm in multiple freezers
located at the Jackson Lab (Maine and California). Live mice can be
recovered by in vitro fertilization and shipped to the UTMB directly
from the Jackson Lab. An additional fee will be applied for this
C. Recovery of Cryopreserved Embryos
To recover live mice from cryopreserved embryos, we will perform embryo transfers of thawed embryos into foster mothers.
Rederivation of Mouse Stocks
can generate specific-pathogen-free mice by embryo transfer from mice
that have been infected with viruses or other pathogenic agents. In
order to acquire mice from non-commercial vendors, the Animal Resources
Center (ARC) may require quarantine or rederivation. We will
super-ovulate multiple females and then isolate the embryos from these
females using sterile techniques. We will then transfer these embryos
into clean foster mothers. Subsequently, the ARC will quarantine and
test the foster mothers and their offspring to verify that they are
If you have other needs in conducting mouse research, please contact us. We will try our best to find a way to assist you.
|Pronuclear injection, standard strain (B6SJLF2)||$4,000|
|Pronuclear injection, non-standard strains||$5,200|
|Electroporation of ES cells||$5,400|
|Embryo cryopreservation/2 sessions||$800|
|For other services, please ask for a quote. Fees are subject to change without notice.|
|Director: Maki Wakamiya, PhD|
|Transgenic Mouse Core Facility|
Institute for Translational Sciences
Animal Resource Center
University of Texas Medical Branch
301 University Blvd.
Galveston, TX 77555-0621