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Antibody Screen (Anti-HLA) (082-0038,082-0111,082-0112(Renal); 082-0059,082-0113,082-0114(Non-Renal); 082-0107,082-0109(Single Antigen Renal); 082-0109,082-0110(Single Antigen Non-Renal))
Test Mnemonic:

PRA, PRA X, PRA T, PRA B, PRAX T, PRAX B

Specimen Requirements:
Collection:

Blood.  10mL red-top (no additive) container

Storage/Transport:

Room temperature; do not refrigerate

Specimen Preparation:

Routine venipuncture

Stability:

Deliver immediately to Tissue Antigen Lab

Causes for Rejection:

Incorrect tube, incorrect label, hemolyzed sample

Reference Range:

N/A

Turnaround Time:

Routine: 14 days; STAT: 1 day; ASAP: 5 days

Methodology:

Luminex microbead assay

 

Performed:

Analysis of patient sera against panel of HLA molecules attached to microbeads for antibody determination.

Lab:

Tissue Antigen Lab

Synonyms:

PRA, Donor Specific Antibodies, DSA

Clinical Indication:

Transplant Candidate (pre-transplant or post-transplant)

Patient Preparation :

 Predialysis (if applicable)

CPT 4 Code:

86829(screen), 86830(ID-Class I), 86831(ID-Class II), 86832(Single Antigen and/or C1q Class I), 86833(Single Antigen and/or C1q Class II)

Note:

Samples can be delivered to the Tissue Antigen Laboratory, Room 4.400, Jennie Sealy Hospital, Monday-Friday, 8 AM to 5:00 PM. Samples received after noon will be processed the next business day.

The request form must contain the following information: patient name, UH #, physician's name, route, and extension.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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