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Gram Stain (8000100644)
Test Mnemonic:

C GS (The gram stain is included in many bacterial culture requests without separate order)

Specimen Requirements:

Collect the sample - aspiration, biopsy, fluid, swab using aseptic technique


Sterile container


Room temperature, refrigerate if transport is delayed


48 hours

Causes for Rejection:

Improper collection, swabs received more than 72 hours after collection. Specimen container unlabeled or labeled incorrectly. No date and time of collection on requisition form.

Reference Range:
Turnaround Time:

Stat - 1 hour, Routine 8-12 hours


Microscopic examination of Gram stained slides


Clinical Microbiology


Direct Gram Stain

Clinical Indication:

 Stain for observation of cellular elements and microorganisms from patient samples.

Patient Preparation :

Cleanse infected site

CPT 4 Code:



A direct gram stain is routinely performed on specimens from most body sites if two swabs are submitted or if fluid, aspirate or tissue is submitted.

Order C WBC for fecal leukocyte exam is requested

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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