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Acid Fast Bacilli (AFB) Culture - Sterile Body Sites (088-0004)
Test Mnemonic:

C AFB

Specimen Requirements:

 Blood and/or Bone marrow aspirates should be inoculated into the Bactec Myco/F lytic (red cap) bottle at the bedside. Other samples are placed in a sterile container.

Collection:

Using aseptic technique collect samples

Container:

Blood and/or Bone marrow aspirates should be inoculated into the Bactec Myco/F lytic (red cap) bottle at the bedside. Other samples are placed in a sterile container.

Minimum Volume:

0.5ml

Storage/Transport:

Room temperature, refrigerate all samples except CSF and Bactec bottles if there are delays in transport.

Stability:

48 hours

Causes for Rejection:

Clotted specimens, swabs,  specimen containers that are unlabeled or labeled incorrectly, no date and time of collection on requisition form.

Reference Range:

No Mycobacteria isolated

Turnaround Time:

Cultures are held 6 weeks, smears are reported the same day as sample processed, positive cultures are reported as soon as growth appears

Methodology:

 Acid fast smear (except for bone marrow), culture for the isolation and identification of Mycobacterium species, antimicrobial susceptibility testing when appropriate.

Performed:

Clinical Microbiology

Synonyms:

AFB Culture; AFB Culture, Bone Marrow; AFB culture, CSF; AFB Culture, Body Fluid; AFB Culture, Tissue

Clinical Indication:

 

Suspected Mycobacterial infections from normally sterile body sites, such as  bone marrow, bone marrow core, CSF, tissues and body fluids

 

 

CPT 4 Code:

Culture: 87116, Smear: 87206, additional if indicated

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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