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Acid fast bacilli Smear (088-0009)
Test Mnemonic:

C AST

Specimen Requirements:
Collection:

Sputum or respiratory samples in sterile container

Container:

Sterile container

Minimum Volume:

3ml

Storage/Transport:

Refrigerate, transport as soon as possible

Specimen Preparation:

Sterile container in biohazard bag

Stability:

72 hours

Causes for Rejection:

Leaking container, samples other than respiratory sources. Specimen container unlabeled or labeled incorrectly. No date and time of collection on requisition form.

Reference Range:

No acid-fast bacilli observed

Turnaround Time:

Stat: 3 hours; Routine: 24-48 hours

Methodology:

Auramine (FA) stain

Performed:

 Clinical Microbiology

Synonyms:

AFB smear; AFB stain; Acid fast Stain

Clinical Indication:

Rapid detection of AFB in respiratory samples

Patient Preparation :

Airborne isolation

CPT 4 Code:

87206

Note:

Clearly indicate that the smear is of STAT priority on lab order. Notify laboratory prior to transport at 772-3310 or 772-1125.

Smears are routinely performed as part of an AFB culture for most specimens. 

The first positive smear from a respiratory specimen is considered a critical value and the LIP will be notified.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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