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Factor VII, Activity (LAB000676)
Test Mnemonic:

FACTOR VII

Specimen Requirements:
Collection:

Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume

Container:

Blue-topped (3.2% sodium-citrate) tube

Minimum Volume:

1 mL of plasma

Storage/Transport:

Shipping/Handling instructions:                                                                           

  1. If delivery time is to be greater than 2 hours from time of draw, centrifuge the specimen for 15 minutes at 2000-2500g.
  2. Transfer plasma to 12x75 plastic tube.
  3. Cap tube and re-spin for 15 minutes at 2000-2500g.
  4. Remove from centrifuge without disturbing any platelet and/or cell pellets that might be on the bottom or sides of the tube.
  5. Carefully transfer plasma into freezer tubes (plastic screw-top cryo-tubes) for testing or freezing and be sure to tape the patient's label onto the tube.
  6. Store transport tube in freezer (-20, or -70C) in an upright position.
 

 

Specimen Preparation:

 

Stability:

Ambient - 2 hours; Refrigerated - Unacceptable; Frozen -20oC – 2 weeks, at -70oC - 6 months 

 

 

If the testing is not completed within 4 hours, platelet-poor plasma should be removed without disturbing the sedimented cells (buff-coat) and frozen at -20oC or below for short-term storage (up to 2 weeks), or -70oC for 6 months.

Causes for Rejection:
  • Clotted and hemolyzed plasma samples are unacceptable and must be redrawn.
  • Wrong tube or anticoagulant.
  • Wrong anticoagulant ratio (over or underfilled tubes).
  • Not centrifuged within the acceptable time after collection.
  • Whole blood or plasma refrigerated or placed on ice prior to testing.
  • Unlabeled.
  • Mislabeled.
 

 

Reference Range:

50 - 129%

 

Infant and child reference ranges have not yet been established at UTMB.

Turnaround Time:

Monday through Friday; 1 hour for STAT; 4 hours for Routine

Methodology:

Optical Clot-Based

Performed:

Samples are accepted 24 hours per day at Sample Management, 7.412 CSW Bldg.

Lab:

Hematopathology

Synonyms:

Factor VII Activity, F7, FVII, Factor VII Deficiency or Factor VII Assay

Clinical Indication:

Measures level of factor VII that can be useful in diagnosing bleeding disorders and liver disease.

Patient Preparation :

Patient must not be on any anticoagulation therapy for 48 hours before blood collection.

CPT 4 Code:

85230

Note:

Interfering substances:  oral contraceptives, anticoagulant therapy, thrombin inhibitors.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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