| KRAS Mutation Detection (8000100522) | |||
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| Test Mnemonic: | KRAS |
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| Specimen Requirements: | |||
| Test Included: | Detection of KRAS codon 12, 13 and 61 mutations |
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| Collection: | Formalin fixed (10 percent neutral buffered formalin) and paraffin embedded tissue sections (prepared in Surgical Pathology). Transport 5 unstained 5-10 micron slides (Minimum is 3 slides) with a matching HE slide marked tumor area. Tumor cell smear/cell block (prepared in Cytopathology) or blood samples may be used when there are enough tumor cells in the sample. |
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| Container: | Slides |
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| Storage/Transport: | Protect specimens from excessive heat. Ship in cooled container during summer months. Transport are room temperature or refrigerated |
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| Specimen Preparation: | Slides are prepared by Surgical Pathology or Cytopathology Labs. |
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| Causes for Rejection: | Specimens with less than 20% tumor cells. Incomplete and/or incorrect specimen identification. |
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| Reference Range: |
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| Turnaround Time: | 7-14 days |
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| Methodology: | PCR, pyro sequencing |
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| Performed: | Molecular Diagnostics Laboratory |
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| Synonyms: | KRAS |
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| Clinical Indication: | May serve as predictive marker for anti-EGFR treatment and tumor prognosis. |
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| Patient Preparation : | Tumor sample |
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| CPT 4 Code: | 88381, 81275, 81276 |
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| Note: | The lab will reflex to BRAF mutation detection test if no KRAS mutation is detected. |
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| When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent. | |||