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Trichomonas Amplified Assay (8000101337)
Test Mnemonic:


Specimen Requirements:

Female - endocervical swab, vaginal swab, liquid Pap (ThinPrep®) collection, urine first catch (first void)

Male - urine first catch (first void)

Test Included:

Trichomonas nucleic acid assay


Swabs - Follow the instructions found with the Aptima® collection kit for endocervical/vaginal specimens.

Urine - Patient should not have urinated at least 1 hour prior to collection.  Patient is instructed to collect a first-catch urine (approximately 20 to 30 mL of the initial urine stream) in a urine collection container free of any preservatives.  Collection of larger volumes of urine may reduce test sensitivity.  Female patients should not cleanse the labial area prior to providing the specimen.

Liquid Pap (ThinPrep®) collection devices may also be submitted for this test.


Aptima® Unisex transport tube (endocervical swab samples only)

Aptima® Vaginal/Multitest transport tube (vaginal swab samples only)

Aptima® Urine transport tube (sterile urine container also acceptable)

Liquid Pap (ThinPrep®) collection container

Minimum Volume:

Urine – 2 mL aliquot from a 20 – 30 mL collection


Refrigerated or room temperature (2 – 30°C)

Do not allow contact with other patient samples in order to avoid any cross contamination.

Specimen Preparation:

Urine - Transfer 2 mL urine first catch (first void) into the Aptima® Urine Specimen Transport tube using the disposable pipette provided in the kit.  The correct volume of urine has been added when the fluid level is between the black fill lines on the urine specimen transport tube label.


Urine samples (2 – 30°C) that are not transferred into and Aptima® Urine transport tube must be received in the laboratory within 24 hours of collection.

Aptima® transport tubes - 30 days refrigerated/room temperature

Liquid Pap (ThinPrep®) containers – 30 days refrigerated/room temperature

Causes for Rejection:

Specimens must meet criteria set forth in the Laboratory Services Specimen Labeling and Requests for Testing policy.

Failure to use Aptima® collection kit for endocervical/vaginal swabs

Samples from non-approved sources

Urine in tube with preservatives

Insufficient quantity of urine for test requirements

Reference Range:


Turnaround Time:

1 - 4 days


Qualitative Transcription-Mediated Amplification


Clinical Microbiology


Trichomonas, Trich, T. vaginalis, Wet Prep, Wet Mount, Vaginitis, Vaginosis

Clinical Indication:

This test is used for the detection of Trichomonas vaginalis in urine first catch (first void), endocervical, vaginal, and liquid Pap (ThinPrep®) specimens

Patient Preparation :

Endocervical or vaginal collection, urine first catch (first void)

CPT 4 Code:



A negative result does not completely rule out infection with T. vaginalis.

Results should be interpreted in conjunction with other clinical data.

This test is intended for medical purposes only and is not valid for the evaluation of suspected sexual abuse or for other forensic purposes.

One sample may be submitted for Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis testing.  ThinPrep® specimens can be used for HPV, Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis testing.

Aptima® collection kits available through Materials Management or by contacting Laboratory Client Services.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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