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Infectious mononucleosis Test, Heterophile Antibody (8000100272)
Test Mnemonic:


Specimen Requirements:

Serum separator tube (SST)


Serum separator tube (SST)

Minimum Volume:

1ml of serum


Refrigerate (2-8oC) up to 48 hours; Samples should be frozen (<-10oC) and tested within 3 months.

Specimen Preparation:

Within two hours of collection, centrifuge.


Refrigerate (2-8oC) up to 48 hours; Samples should be frozen (<-10oC) and tested within 3 months.

Causes for Rejection:

Improper collection, gross hemolysis, bacterial contamination, lipemic sample. Specimen container unlabeled or labeled incorrectly. No date and time of collection or collector information on order.

Reference Range:


Turnaround Time:

Galveston Campus: Test is performed daily

League City and Clear Lake Campus: Emergency Department 1 hour, Outpatient 4 hours


Color Immunochromatographic dipstick


Clinical Microbiology


Mono test; Mono Spot; Mononucleosis Test; Infectious mononucleosis heterophile antibody

Clinical Indication:

Suspected mononucleosis disease (Epstein Barr virus)

Patient Preparation :

Routine venipuncture

CPT 4 Code:



Sensitivity is reduced in children under 12 years of age.   If the mono test (heterophile antibody) is negative on a patient less than four years of age, an Epstein Barr virus VCA antibody IgM test, VCA IgG, and EBNA antibody tests are suggested to rule out mononucleosis in this age group.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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