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Anti-Sm (SMITH) Antibody (8000100305)
Test Mnemonic:
Specimen Requirements:

Serum separator tube (SST)


Serum separator tube (SST)

Minimum Volume:

1 mL of serum


Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Specimen Preparation:

Within 2 hours of collection, centrifuge


Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Causes for Rejection:

Improper collection, gross hemolysis.  Specimen container unlabeled or labeled incorrectly. No date and time of collection on requisition form.

Reference Range:

Positive - Antibody detected. 

Negative - No antibody detected

Turnaround Time:

Test is performed daily, Sunday through Friday


Multiplexing bead immunoassay


Clinical Microbiology


Smith Antibody; Sm Antibody; ENA Antibodies

Clinical Indication:

Autoimmune disease testing

CPT 4 Code:



Smith antibody is very specific for systemic lupus erythematosus (SLE) but only occurs in 30-35% of SLE cases. The presence of antibodies to Smith is often associated with renal disease.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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