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Factor 2 G20210A Mutation ([LAB001988])
Test Mnemonic:


Specimen Requirements:

Whole Blood


EDTA (lavender), K2EDTA (pink), Sodium Citrate (blue), or Acid Citrate Dextrose (yellow) anticoagulant tube

Minimum Volume:

3 mL of whole blood


Refrigerate at 2-8C up to 15 days.

Specimen Preparation:

Do NOT centrifuge.


Refrigerate at 2-8C up to 15 days.

Causes for Rejection:

Specimens that have been centrifuged. Insufficient quantity, gross hemolysis, lipemia. Specimen container unlabeled or labeled incorrectly. No date and time of collection or collector information on order.

Reference Range:


Turnaround Time:

3 days


Reverse transcriptase polymerase chain reaction


Clinical Microbiology


F2, coagulation factor II, FII, thrombin, prothrombin, F2 G20210A mutation, Factor II 20210 mutation

CPT 4 Code:



Factor 2 Comment:

Phenotype Characteristics: Thrombophilia due to production of too much coagulation factor 2 (F2, also called prothrombin)

Incidence: Approximately 2-5 percent of Caucasians and 0.3 percent of African Americans are heterozygous; about 1 in 10,000 individuals are homozygous with two copies of the mutation. 

Penetrance: The risk of thrombosis is increased 2-4 fold for heterozygotes and further increased for homozygotes.

Mutation tested: F2 c.20210G>A (G20210A).

Clinical Sensitivity for Venous Thrombosis: Approximately 10%.

Methodology: Polymerase Chain Reaction and fluorescence monitoring

Limitations: The performance of this assay has not been evaluated with samples from pediatric patients. 

Counseling and informed consent are recommended for genetic testing.

References: OMIM:176930


When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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