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VITAMIN D 25-HYDROXY (087-0376)
Test Mnemonic:


Specimen Requirements:

7mL of blood in Red Top Serum Tube or SST Gold Top Tube. (Min.: 3mL of blood).


Delivered to Sample Management within 2 hours of collection.    

Specimen Preparation:

Allow specimen to clot completely at room temperature. Centrifuge to separate serum from cells within 2 hours of collection. Transfer serum from collection tube to labeled plastic transport tube.   


Refrigerated for ≤ 7 days; Frozen for ≤ 4 weeks. (Avoid repeated freeze/thaw cycles).     

Causes for Rejection:

Incomplete and/or incorrect sample identification, improper storage/transport, gross hemolysis and lipemia.                                            

Reference Range:

25-80 ng/mL

Turnaround Time:

M-F: within 24 hours




High Volume Chemistry

Clinical Indication:

Vitamin D deficiency

CPT 4 Code:


When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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