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HIV 1/2 Ag-Ab with Reflex (LAB001997)
Test Mnemonic:

HIV Ag-Ab Multiplex

Specimen Requirements:
Test Included:

Samples are screened for antibodies to HIV-I and HIV-II and for HIV-I P24 antigen. If the screening test is reactive, a confirmation test (HIV 1/2 Supplementation) is performed for HIV-I and HIV-II as appropriate.


Serum separator tube (SST) (Preferred), Plasma separator tube (PST) or red stopped vacutainer tube.


Deliver to Sample Management within 2 hours of collection.

Specimen Preparation:

Allow specimen to clot completely at room temperature. Centrifuge to separate serum from cells within 2 hours of collection.


24 hours at  room temperature.  Refrigerated for 7 days.

Causes for Rejection:

Specimen from individuals less than 2 years of age.  Pour over tube, specimens containing particulate matter, specimens containing sodium azide, heat-inactivated specimens, bacterial contamination ,incomplete request/consent forms, incomplete and/or incorrect sample identification, improper storage/transport, and gross hemolysis and lipemia, gross hemolysis, leaking container.

Reference Range:


Turnaround Time:

Routine: 8 hours, ASAP: 4 hours.  Confirmation of positives requires 3 – 7 days 




HVL Chemistry

Clinical Indication:

Possible HIV-1 or HIV-2 infection.

CPT 4 Code:



This test has not been validated for individuals less than 2 year of age.

A consent form signed by patient, witness, and ordering physician must be completed for each specimen for inpatient testing and placed in the patient's chart. For non-UTMB patients, the physician is responsible for obtaining written consent and performing appropriate counseling for the test. To ensure irrefutable identification, the laboratory strongly recommends that the original collection tube be submitted to the laboratory for this test.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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