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Clostridioides (Clostridium) difficile toxin (8000100147)
Test Mnemonic:


Specimen Requirements:

Pseudomembranous colitis; Detection of toxigenic Clostridioides (Clostridium) difficile from "watery" fecal samples.


Submit specimen in a sterile, spillproof container. Only specimens of soft, loose, or watery consistency are tested. 


Sterile, spillproof container. 

Minimum Volume:

2.0 mL


Store/transport specimen refrigerated, 2-8°C.  

Specimen Preparation:

Transfer feces to a clean, spillproof container 


For optimal results, specimens must be refrigerated and tested within 72 hours of collection.

Causes for Rejection:

Formed stool specimens, insufficient quantity, sample on culturette, stool in preservatives, specimen container unlabeled or mislabeled, no date and time of collection on form.

Testing is not repeated within 7 days. Duplicate requests (negative or positive) rejected if collected within 7 days. 

Children <24 months old will only be performed with Pediatric ID or Pediatric GI approval.

Fecal specimens received in formalin-based or alcohol-based fixatives.

Reference Range:


Turnaround Time:

Specimens are batch tested three times within a day.


EIA membrane assay, PCR (for reflex tests only)

Discordant results will be reflexed to PCR for confirmatory testing


Clinical Microbiology


Pseudomembranous colitis test; C. difficile toxin assay; C. difficile toxigenic assay; Clostridioides (Clostridium)difficile test; Clostridioides (Clostridium) difficile toxin test; Clostridioides (Clostridium) difficile cytotoxin assay.

Clinical Indication:

Aid in the diagnosis of Clostridioides (Clostridium) difficile related disease

Patient Preparation :

This test is performed on patients with diarrhea.

CPT 4 Code:

87449, 87324, 87493 (if reflexed to PCR)

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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