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Anti-Ribonucleoprotein (RNP) Antibody (8000100338)
Test Mnemonic:
Specimen Requirements:

Serum separator tube (SST)

Red top serum tube with no additive


Serum separator tube (SST)

Red top serum tube with no additive

Minimum Volume:

1 mL of serum


Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Specimen Preparation:

Within 2 hours of collection, centrifuge. Serum collected in a red top tube should be removed from the red cells if testing will be delayed.


Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Causes for Rejection:

Improper collection, gross hemolysis.  Specimen container unlabeled or labeled incorrectly. No date and time of collection on requisition form.

Reference Range:

Positive - Antibody detected. 

Negative - No antibody detected

Turnaround Time:

Test is performed in batch, once per day, six days a week.


Multiplexing bead immunoassay


Clinical Microbiology


RNP, Ribonucleoprotein Antibody; ENA Antibodies

Clinical Indication:

Autoimmune disease testing

CPT 4 Code:



RNP antibody is seen in 95-100% of mixed connective tissue disease and is considered specific for this syndrome if other antibodies are negative; RNP is also present in 20-30% of systemic lupus erythematosus and 15-25% of progressive systemic sclerosis. RNP antigens also contain epitopes that are immunologically indentical to free Smith antigens, therefore, the Smith antibody response must be considered when interpreting RNP results.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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