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HSV 1&2 NAAT (8000200116)
Test Mnemonic:
Specimen Requirements:

Swab of lesion.  Place swab in viral transport medium (VTM) or universal transport medium (UTM).

Collect CSF via lumbar puncture into sterile container
Test Included:

Herpes simplex virus type 1, Herpes simplex virus type 2
Collection:

Swab Collection-Collect lesions by swabbing the lesion base. The swab is put into UTM.

Standard collection and handling procedures used to collect CSF via lumbar puncture.

 
Container:

Swab-Viral Transport Medium (VTM) or Universal Transport Medium (UTM)

CSF-sterile container

 
Minimum Volume:

Swab-3ml of UTM

CSF-0.25ml

 
Storage/Transport:

Specimen should be delivered to the lab ASAP on ice. 

 
Specimen Preparation:

Lumbar Puncture

  1. Insert a needle with stylet at the L3-L4 or L4-L5 or L5-S1 interspaced.  When the subarachnoid space is reached, remove the stylet and spinal fluid will appear in the needle hub.
  2. Slowly drain the CSF into the sterile leak-proof tube.

 
Stability:

Up to 7 days prior to processing when stored at 2-8C or -20C. 

>7 days must be frozen -70C
Causes for Rejection:

Swabs-Improper collection, use of wooden swabs,

Swab and CSF-unlabeled or mislabeled samples, no date and time of colelction or no collector information in order.

 
Reference Range:

Negative 
Turnaround Time:

Test is performed once daily.
Methodology:

Nucleic Acid Amplification Test
Performed:

Clinincal Microbiology 
CPT 4 Code:

87529
When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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