| Complete Blood Count with Differential, Reflex to Peripheral Smear Review (8000101309) | |
|---|---|
| Test Mnemonic: | CBC with Diff |
| Specimen Requirements: | |
| Collection: | Routine venipuncture; Collect in EDTA (lavender tube). Micro-collection is acceptable. The specimen should be collected to the appropriate fill volume stated on the collection tube. The minimum volume required is 90% of the stated tube volume. |
| Container: | EDTA (lavender tube). Micro-collection tube is acceptable. |
| Minimum Volume: | 90% of the stated tube volume. Micro-collections minimum 250uL, maximum 500uL. |
| Storage/Transport: | Room Temperature 8 hours. If testing cannot be completed within 8 hours, sample must be refrigerated at 2-8° C. |
| Specimen Preparation: | ECMO specimens should be delivered directly to the Hematopathology lab. |
| Stability: | Stable Refrigerated at 2-8° C for 48 hours. |
| Causes for Rejection: | Clotted or presence of fibrin; QNS (quantity not sufficient/underfilled); overfilled; gross hemolysis; samples drawn above IV line or contaminated with IV fluid; Mislabeled or underlabeled; incorrect storage temperature; stability exceeded; incorrect anticoagulant. |
| Reference Range: | By report (reports may vary based on instrumentation, patient age and sex). |
| Turnaround Time: | STAT/ASAP: 1 hour; Routine: 8 hours |
| Methodology: | Automated, multi-parameter counting of red blood cells (RBC) and platelets using electronic resistance detection enhanced by hydrodynamic focusing. The hematocrit is measured utilizing cumulative pulse height detection. Hemoglobin is measured using a sodium lauryl sulfate method. WBC count, differential and nucleated red blood cells are evaluated using flow cytometry. |
| Performed: | Samples are accepted 24 hours per day. |
| Lab: |
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| Synonyms: | CBC with differential, CBC W/DIFF, CBC |
| CPT 4 Code: | 85025. If reflexed additional CPT codes may apply; refer to reflexed test code for applicable codes. Additional charges apply. |
| Note: | Critical Values: WBC ≤ 2.0 x 103 /uL Hemoglobin: ≤ 7.0 g/dL (age <2 yrs. old), ≤5.0 g/dL (age ≥2 yrs. old) Hematocrit: ≤ 21% (age <2 yrs. old), ≤15.0% (age ≥2 yrs. old) Platelets: ≤ 50 or ≥ 1000 x 103 /uL Limitations and Interfering Substances: Clots or fibrin strands; cryoprotein; cryoglobulin; leukocytosis >100,000/uL; platelet clumps; giant platelets, hemolysis; electrolyte imbalances; warm or cold agglutinins; lipemia; abnormal protein; severe diabetes; uremia. |
| When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent. | |