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HLA-ABC Typing - Renal (082-0037(Cytotoxic), 082-00103(Molecular))
Test Mnemonic:

HLA R, ABC

Specimen Requirements:
Collection:

Blood.  10mL blood (with normal white cell count)

Container:

Pale Yellow (Acid Citrate Dextrose) collection tube

Minimum Volume:

10mL

Storage/Transport:

Room temperature; do not refrigerate

Specimen Preparation:

Routine Venipuncture

Stability:

Deliver immediately to Tissue Antigen Lab

Causes for Rejection:

Sample more that 72 hours old, incorrect tube, incorrect label, insufficient white cell count, poor viability.

Reference Range:

N/A

Turnaround Time:

Routine: 4 days;  STAT: 8 hours

Methodology:

Cytotoxic and/or DNA typing for HLA A, B, and C loci

Performed:

Identification of patient's HLA A, B, and C locus antigens

Lab:

Tissue Antigen Laboratory

Clinical Indication:

Transplantation

Patient Preparation :

Predialysis

CPT 4 Code:

86813(Cytotoxic);  81372 or 81370(Molecular)

Note:

Samples can be delivered to the Tissue Antigen Laboratory, Room 3.350J, Children's Hospital, Monday-Friday, 8 AM to 5:00 PM. Samples received after noon will be processed the next business day.

Schedule 24 hours in advance. The request form must contain the following information: patient name, UH #, physician's name, route, and extension.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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